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Method for detecting free glucosamine in amino-oligosaccharin pesticide

A technology for amino oligosaccharin and free amino groups, which is applied in the field of detection of free glucosamine in amino oligosaccharin pesticides. It can solve the problems of insufficient separation, quantitative research of free glucosamine, and easy interference in quantification. Application value, accurate and reliable test results, and the effect of accurate judgment

Active Publication Date: 2022-02-25
陈炜瑶
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are relatively few studies on the quantitative methods of free glucosamine in oligosaccharins at home and abroad, such as Li Juan et al. Glucose is susceptible to the interference of chitosan and chitotriose, the degree of separation cannot meet the requirements, and the quantification is easily disturbed, especially when the content of free glucosamine is low; Liu Yanxia et al. Method Discussion) using spectrophotometer to detect the content of amino oligosaccharin in the amino oligosaccharin aqueous preparation, Shao Bo et al. saccharin) content, no quantitative research on free glucosamine

Method used

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  • Method for detecting free glucosamine in amino-oligosaccharin pesticide
  • Method for detecting free glucosamine in amino-oligosaccharin pesticide
  • Method for detecting free glucosamine in amino-oligosaccharin pesticide

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Experimental program
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Embodiment

[0022] 1 Experimental part

[0023] 1.1 Instruments and reagents

[0024] Thermo Scientific TM Dionex TM ICS-6000 ion chromatography system, including: ICS-6000 quaternary gradient pump module, ICS-6000DC module (including amperometric detector), working electrode: Au, reference electrode: Ag / AgCl; ThermoScientific TM Dionex TM AS-AP autosampler; Thermo Scientific TM Dionex TM Chromeleon TM Chromatography workstation.

[0025] Glucosamine standard substance (purity ≥ 98% BOC Sciences); NaOH solution (Fisher, mass fraction 50%); parent drug of oligosaccharin (content ≥ 85%, Shenzhen Nuopuxin Agrochemical Co., Ltd.).

[0026] 1.2 Chromatographic conditions

[0027] Guard column: DionexCarboPac PA100, 4mm×50mm

[0028] Analytical column: DionexCarboPac PA100, 4mm×250mm

[0029] Amino trap column: DionexAminoTrap Column, 4mm×50mm

[0030] The eluent and gradient conditions are shown in Table 1

[0031] Column temperature: 30°C

[0032] Detector box temperature contr...

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Abstract

The invention relates to the technical field of pesticide detection and analysis, in particular to a method for detecting free glucosamine in amino-oligosaccharin pesticide. The invention establishes a method for detecting free glucosamine in amino-oligosaccharin parent drug and water aqua through efficient anion exchange-integral pulse, DionexAminoTrap Column (amino capture column) and CarboPacPA100 anion exchange columns are used as separation columns, a 17mmol / L NaOH solution is used for eluting the target free glucosamine, a 200mmol / L NaOH solution is used for flushing amino-oligosaccharin, the flow rate is 1.0 mL / min, and the total analysis time is 30 minutes; under the optimal separation condition, the interference of the amino-oligosaccharin on the glucosamine can be eliminated, the detection limit of the glucosamine can reach 0.01 mg / L (S / N = 3), the standard linear range is 0.02-10 mg / L, and the correlation coefficient is 0.99994. When commercially available amino-oligosaccharin mother drugs and water aqua are detected, the relative standard deviation (RSD) of three parallel samples is less than 1.9%, and the addition recovery rate is between 90% and 99%.

Description

technical field [0001] The invention relates to the technical field of pesticide detection and analysis, in particular to a method for detecting free glucosamine in amino-oligosaccharin pesticides. Background technique [0002] Amino oligosaccharins (also known as oligochitosaccharides) refer to oligosaccharides linked by D-glucosamine (GIcN) with β-1,4 glycosidic bonds. Inhibitory effect affects the germination of fungal spores, induces changes in mycelial morphology, and changes the biochemical reactions in mycelial cells; it can also stimulate gene expression in plants to produce chitinase and glucanase with disease resistance , phytoalexin and PR protein, etc., and has cell activation effect, which is helpful for the recovery of damaged plants, promotes strong roots and seedlings, enhances stress resistance of crops, and promotes plant growth and development. [0003] Amino oligosaccharins have both bactericidal and nutritional functions, and have the characteristics of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/34G01N30/60G01N30/64G01N30/54G01N30/32
CPCG01N30/02G01N30/34G01N30/60G01N30/64G01N30/54G01N30/32G01N2030/324Y02A50/30
Inventor 陈炜瑶吴国权林昊陈翠玲柯立坚戴柏桉陈树清高凡钦
Owner 陈炜瑶
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