Use of an opioid molecule for treating dry eye and eyes suffering from allergies
A dry eye, pharmaceutical technology in the field of use of opioid substance molecules for the treatment of dry eye and eyes with allergies, capable of addressing the absence of eye allergies and their consequences
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[0058] To investigate this effect on CFTR modulation, the inventors used the Ussing chamber method, invented by the Dane Hans H. Ussing in the late 1950's. This technique allows the study of ion exchanges through the epithelium as they allow the tissue of interest to be kept alive for hours under controlled temperature and media conditions. The positioning of the tissue between the two half-chambers allows to define an apical compartment (corresponding to the lumen of the organ) and a basolateral compartment (corresponding to the blood compartment) and to study the exchange between these two compartments through the tissue.
[0059] This technique is commonly used, and it is particularly suitable for pharmacological approaches to ion transport and to search for molecules of therapeutic interest in the context of ion secretions from epithelial cells. It involves measuring the transepithelial current (called short circuit and denoted Ise). Ise is expressed in amperes relative t...
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