Circular RNA marker and application thereof in diagnosis and prediction of recurrent abortion pathology pregnancy

A technique for recurrent miscarriage, pathological pregnancy, applied in the field of biotechnology and medicine, which can solve the problem of poorly understood diagnostic and/or predictive methods of RSA

Pending Publication Date: 2022-03-01
上海市生物医药技术研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In summary, from the available research it can be seen that little is known about the diagnostic and / or prognostic methods for RSA

Method used

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  • Circular RNA marker and application thereof in diagnosis and prediction of recurrent abortion pathology pregnancy
  • Circular RNA marker and application thereof in diagnosis and prediction of recurrent abortion pathology pregnancy
  • Circular RNA marker and application thereof in diagnosis and prediction of recurrent abortion pathology pregnancy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0138] Real-time fluorescent quantitative PCR detection of expression level of circular RNA hsa_circ_0007291 in villous tissue

[0139] According to the above recruitment criteria, 26 cases of recurrent abortion patients and 24 cases of induced abortion patients were collected. The RNA of the above-mentioned villous tissues was extracted respectively, and the expression level of circular RNA hsa_circ_0007291 in the two groups of 50 cases of villous tissues was detected by real-time fluorescent quantitative PCR. According to the detection results, the relative expression of circular RNA hsa_circ_0007291 in the two groups of villi tissues was calculated and statistically analyzed.

[0140] Adopting SEQ ID No:2 and 3 is the length of the amplified product that primers obtain is 152bp, and its sequence such as SEQ ID No:5 and through sequencing verification Figure 7 shown. Sequence analysis shows that the amplified product corresponds to RNA hsa_circ_0007291 (SEQ ID No: 1 or 4)...

Embodiment 2

[0145] Real-time fluorescent quantitative PCR detection of expression level of serum circular RNAhsa_circ_0007291

[0146] According to the above recruitment criteria, 12 serum samples of recurrent abortion patients and 13 serum samples of induced abortion patients were collected. The RNA of the above serum samples was extracted respectively, and the expression level of circular RNA hsa_circ_0007291 in 25 cases of villous tissues of the two groups was detected by real-time fluorescent quantitative PCR. According to the detection results, the relative expression of circular RNA hsa_circ_0007291 in the serum samples of the two groups was calculated and statistically analyzed.

[0147] Adopting SEQ ID No:2 and 3 is the length of the amplified product that primers obtain is 152bp, and its sequence such as SEQ ID No:5 and through sequencing verification Figure 7 shown.

[0148] The result is as Figure 4 As shown, the relative expression level of circular RNA hsa_circ_0007291 i...

Embodiment 3

[0152] Kits for the detection of recurrent abortion (RSA)

[0153] A kit is prepared, which contains the following primers and instructions for specifically amplifying circular RNA hsa_circ_0007291:

[0154] 5'-CAGCTCGCTGCTCATGTCT-3', SEQ ID NO: 2; and

[0155] 5'-TCTACTGCCAGGAGGTTCTTG-3', SEQ ID NO:3.

[0156] The instructions indicate that the kit is used for (a) diagnosing RSA, and / or (b) predicting the occurrence of RSA.

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Abstract

The invention relates to a circular RNA marker and application thereof in diagnosis and prediction of recurrent abortion pathological pregnancy. Specifically, the invention provides application of the circular RNA marker circular RNA hsacirc0007291 and a detection reagent thereof in diagnosis and prediction of the recurrent abortion (RSA), including preparation of a diagnostic reagent or a kit for diagnosing / predicting the RSA. Researches show that the circular RNA marker disclosed by the invention can be used as a marker for diagnosing RSA and predicting RSA generation, and has high sensitivity and specificity.

Description

technical field [0001] The present invention relates to the fields of biotechnology and medicine, more specifically, to a circular RNA marker and its application in diagnosing and predicting recurrent miscarriage RSA pathological pregnancy. Background technique [0002] Recurrent spontaneous abortion (RSA) refers to women who have at least 2 spontaneous abortions with the same sexual partner [1] . The vast majority of RSA occurs in the first trimester, usually within 10 weeks of gestation [2] . The etiology of RSA is diverse and complex, among which the known causes include abnormal endocrine function, anatomical factors, genetic factors, immunological factors, infectious diseases, advanced age of both parents, etc. About 50% of RSA has unknown causes [3] . However, clinical research on how to diagnose and / or predict the occurrence of RSA is still blank. [0003] Circular RNAs (circular RNAs, circRNAs) are a new type of competitive endogenous non-coding RNAs with regula...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/178C12Q2600/158
Inventor 刘军伟杜晶徐建华张兆奉顾艳朱伟强
Owner 上海市生物医药技术研究院
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