Muscovy duck stress rapid detection method and Muscovy duck stress prevention and control method

A detection method, Muscovy duck technology, applied in the field of livestock and poultry breeding, can solve the problems of lack of effective, rapid and accurate detection of Muscovy duck stress response, reduce the quality of Muscovy duck meat, and endanger the health of Muscovy duck, so as to prevent the decline of meat quality , Improving growth performance, reducing the effect of morbidity and mortality

Pending Publication Date: 2022-03-08
赣州市畜牧水产研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention aims to solve the problem that when Muscovy ducks are in stress response, it will endanger the health of Muscovy ducks and reduce the quality of Muscovy duck meat. The prior art lacks an effective, fast and accurate method for detecting the stress response of Muscovy ducks. The rapid and accurate detection of the stress response of Muscovy ducks is realized by using fluorescent quantitative PCR to detect the expression of Hsp70, which realizes the rapid and accurate detection of stress in Muscovy ducks, and the test results can be obtained in the fastest 1 hour. It can be accurately known by the present invention at the initial stage, that is, when the expression level of Hsp70 is higher than 0.3ng / ml, it is in a stress response

Method used

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  • Muscovy duck stress rapid detection method and Muscovy duck stress prevention and control method
  • Muscovy duck stress rapid detection method and Muscovy duck stress prevention and control method
  • Muscovy duck stress rapid detection method and Muscovy duck stress prevention and control method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Step 1: Design a pair of primers for detecting Hsp70 mRNA of muscovy duck stress, primer F: 5'-TAGTCACAAAGCCAAAAACCC-3', R: 5'-CCCACTGTTGCCATCCC-3'.

[0038] Step 2: Using PCR technology to amplify the rapid detection sequence of Muscovy duck is:

[0039] TAGTCACAAA°GCCAAAACCC°AAAGTTGAAC°TCCCAAAGGA°GGAGCAGAAA°CCAGCTGAAC°CGAATGGACC°AGTAGAAGGC°CAGGGGGATG°GCAACAGTGG°G, the total length is 101bp.

[0040]Step 3: Optimizing the Hsp70 mRNA fluorescent quantitative PCR method, wherein the amplification system is: the total reaction volume is 20 μL, of which 2×PerfectStartTM Green qPCR SuperMix is ​​10 μL, HSP 70-F (10 μM) is 0.4 μL, HSP 70-R (10 μM) 0.4 μL, Passive Reference Dye (50×) (optional) 0.4 μL, cDNA 5 μL, Nuclease-free Water 3.8 μL.

[0041] Amplification conditions: Pre-denaturation at 94°C for 10 minutes, amplification of the target gene 32 times at 94°C, 10s, 58°C, 30s, 72°C, 32s, and then analysis of melting curves: 94°C for 15sec, 60°C for 1min, 94°C for 15sec ...

Embodiment 2

[0053] The difference between this example and Example 1 is: 24 hours before the detection, anti-stress drugs were fed, 1.5-3.5% mixed material was fed, and continuous feeding was carried out for 5 days. Observe the morbidity, mortality and meat quality of Muscovy duck within 4 weeks.

[0054] The preparation method and composition of the anti-stress medicine are as follows: it includes selenium-enriched earthworm liquid, selenium-enriched feed mulberry leaves, feed mulberry leaves, orange peel, orange fruit, stevia sugar dregs, herbal tea dregs, and andrographis paniculata. After crushing orange peel, orange fruit, stevia residue, and herbal tea residue into 5mm-7mm sizes, mix them according to the mass fraction of 3:4:5:2, and then add them to the feed mulberry leaves and Andrographis paniculata according to the ratio of 5:5. The leaves and Andrographis paniculata were cut into 2×3cm size, the mass fraction was 7:3, and yeast and lactic acid bacteria were added for closed an...

Embodiment 3

[0056] The difference between this example and Example 2 is that the antistress drug only consists of antistress additive A.

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Abstract

The invention relates to the technical field of livestock and poultry breeding, in particular to a muscovy duck stress rapid detection method and a muscovy duck stress prevention and control method. The invention aims to solve the problems that when the Muscovy ducks are in stress reaction, the health of the Muscovy ducks is harmed, meanwhile, the meat quality of the Muscovy ducks is reduced, and a method for effectively, quickly and accurately detecting the stress reaction of the Muscovy ducks is lacked in the prior art. The rapid detection method for the stress of the muscovy duck comprises the following specific steps: acquiring blood of the muscovy duck, extracting RNA (Ribonucleic Acid), then carrying out reverse transcription on cDNA (Complementary Deoxyribonucleic Acid), and detecting the expression quantity of Hsp70 through a fluorescent quantitative PCR (Polymerase Chain Reaction) method. According to the present invention, the rapid and accurate detection of the Muscovy duck stress reaction is achieved, the fluorescence quantitative PCR is adopted to detect the expression quantity of the Hsp70, the rapid and accurate Muscovy duck stress detection is achieved, the detection result can be obtained in the fastest 1 h, and the Muscovy duck can be accurately known through the method when the Muscovy duck is in the early stage of the body stress Muscovy duck, that is, the Muscovy duck is in the stress reaction when the expression quantity of the Hsp70 is higher than 0.3 ng / ml.

Description

technical field [0001] The invention relates to the technical field of livestock and poultry breeding, in particular to a rapid detection method for Muscovy duck stress and a method for its prevention and control. Background technique [0002] Animal stress response is a kind of non-specific response produced by animal body when it is stimulated by adverse factors such as environmental change and disease infection. An appropriate amount of stress response is the body's adaptive response to external changes, which helps to improve the body's immunity; but excessive stress response will cause serious damage to the body, not only affecting the health of the body, but also affecting the quality of livestock and poultry meat. Seriously affect and reduce meat quality. At present, in Muscovy duck breeding, high heat and high humidity in the south, heavy metal pollution in feed or drinking water, long-distance transportation, and diseases are all important factors that cause the st...

Claims

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Application Information

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IPC IPC(8): C12Q1/6851C12Q1/6888A61K36/82A61P39/00A61K33/04A61K35/62A61K31/704
CPCC12Q1/6851C12Q1/6888A61K33/04A61K35/62A61K36/605A61K36/752A61K31/704A61K36/82A61K36/19A61P39/00C12Q2600/124C12Q2600/158C12Q2600/166A61K2236/19C12Q2531/113C12Q2563/107C12Q2545/114A61K2300/00
Inventor 雷小文李建军钟云平刘珍妮张强孔智伟谢华亮
Owner 赣州市畜牧水产研究所
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