Method for screening characteristic marker from bee pollen extract and application of characteristic marker in identification of bee pollen

A technology of bee pollen and extract, which is applied in the application field of identifying bee pollen, can solve the problems that cannot really reflect the quality level, and achieve excellent whitening effect

Pending Publication Date: 2022-03-08
HUAZHONG AGRI UNIV
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Problems solved by technology

Because bee pollen contains a variety of compounds and has holistic characteristics, the determination of a singl

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  • Method for screening characteristic marker from bee pollen extract and application of characteristic marker in identification of bee pollen
  • Method for screening characteristic marker from bee pollen extract and application of characteristic marker in identification of bee pollen
  • Method for screening characteristic marker from bee pollen extract and application of characteristic marker in identification of bee pollen

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[0026] The specific embodiment provides a method for screening out characteristic markers from bee pollen extract, comprising the following steps:

[0027] S1. Use high-performance liquid chromatography tandem high-resolution mass spectrometry technology to analyze the bee pollen extract to obtain relevant atlas data; in the high-performance liquid chromatography tandem high-resolution mass spectrometry technology, set the flow rate of the chromatographic column to be 0.3 mL / min; Box at 40°C; mobile phase: 0.13% formic acid (A), acetonitrile (B). The gradient elution conditions were: 0min, 5%B; 3min, 5%B; 20min, 30%B; 25min, 60%B; 27min, 5%B; 30min, 5%B, the injection volume was 2μL, and the detection wavelength was 280nm ; Mass spectrometry analysis adopts the form of electrospray ionization, positive ion mode; mass spectrometry conditions are: ion spray voltage is 5500mV, temperature is 550 ℃, ion source gas is 155; , the collision energy is 35V, and the cluster potential i...

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Abstract

The invention discloses a method for screening characteristic markers from bee pollen extracts and application of the characteristic markers in identification of bee pollen, and belongs to the technical field of bee pollen. The method for screening the characteristic marker from the bee pollen extract comprises the following steps: S1, analyzing the bee pollen extract by adopting a high performance liquid chromatography-tandem high resolution mass spectrometry technology to obtain related map data; s2, processing the atlas data to obtain data corresponding to peaks; s3, screening data corresponding to peaks, and removing data with p greater than 0.05 and data without two-dimensional mass spectrum to obtain a data set; s4, performing multivariate statistical analysis on the data set to obtain differential compounds; and S5, analyzing the correlation between the differential compound and tyrosinase inhibition activity, and screening out a characteristic marker. The invention also comprises application of the characteristic marker in identification of bee pollen. The screened characteristic marker can be used for accurately and quickly judging whether the bee pollen to be detected has an excellent whitening effect or not.

Description

technical field [0001] The invention relates to the technical fields of functional food, cosmetics and health products, in particular to a method for screening characteristic markers from bee pollen extracts and the application of the characteristic markers in identifying bee pollen. Background technique [0002] Tyrosinase is a key enzyme in the formation of human melanin. Tyrosinase hydroxylates tyrosine to produce dopamine, which is oxidized to dopaquinone, which forms melanin under non-enzymatic conditions. Excessive melanin will lead to hyperpigmentation of the human body, affect the color of human skin, and also cause skin diseases such as age spots, freckles, and chloasma. Inhibition of tyrosinase activity is often used as an evaluation index for whitening products. The higher the activity of inhibiting tyrosinase, the better the whitening effect. [0003] Bee pollen is a granular substance made from a mixture of plant pollen, nectar, and bee saliva. Bee pollen co...

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Application Information

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IPC IPC(8): G01N30/02G01N30/32G01N30/54G01N30/34G01N30/72G01N30/86
CPCG01N30/02G01N30/32G01N30/54G01N30/34G01N30/72G01N30/8679G01N2030/324
Inventor 鲁群张星星喻美华刘睿
Owner HUAZHONG AGRI UNIV
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