Lactobacillus reuteri CCFM1132 for relieving hyperuricemia and ulcerative colitis and application
A technology of CCFM1132 and Lactobacillus reuteri, applied in the field of microorganisms, can solve problems such as unclear pathogenesis, many side effects, and limited efficacy of therapeutic drugs, so as to relieve hyperuricemia and gout, improve disease symptoms, and reduce hyperuricemia The effect of blood and gout
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Embodiment 1
[0051] Example 1: Screening of Lactobacillus reuteri CCFM1132
[0052] (1) Isolation and screening of Lactobacillus
[0053] (1) Take 1g of fresh feces of healthy adults. After gradient dilution, it was applied to LBS medium supplemented with 1% nystatin, and placed in a constant temperature incubator at 37°C for 48 hours.
[0054] (2) After cultivation, according to the color, size, and edge shape of the colony, use an inoculation loop to pick up the colony and streak it for purification.
[0055] (3) The obtained colonies were subjected to Gram staining and catalase analysis.
[0056] (4) Retain Gram-positive bacilli and catalase-negative bacteria.
[0057] (2) Molecular biological identification of Lactobacillus
[0058] (1) Single bacterial genome extraction
[0059] (A) cultivating the lactobacilli screened in step (1) overnight;
[0060] (B) Take 1 mL of the bacterial suspension cultivated overnight in a 1.5 mL centrifuge tube, centrifuge at 10000 r / min for 2 min, ...
Embodiment 2
[0076] Example 2: Lactobacillus reuteri CCFM1132 has no toxic side effects on KunMing mice
[0077] Lactobacillus reuteri CCFM1132 was cultured in MRS medium at 37°C, the bacteria were collected, and the bacteria were resuspended in 100g / L skim milk solution to make a concentration of 4.0×10 9 CFU / mL bacterial suspension. Take 12 healthy male KunMing mice with a body weight of about 24-32g. After adapting to the environment for a week, they are divided into CCFM1132 group and control group. The 100g / L skim milk solution that does not contain Lactobacillus reuteri CCFM1132 was observed for a week, and the death and body weight were recorded.
[0078] The results of these tests are listed in Table 1. These results indicate that feeding concentrations of 1×10 9 CFU / Lactobacillus reuteri CCFM1132 had no significant impact on mice, no significant change in body weight, and no death. The appearance of the mice had no obvious pathological symptoms.
[0079] Table 1 Changes in bo...
Embodiment 3
[0082] Example 3: Lactobacillus reuteri CCFM1132 reduces serum uric acid levels in hyperuricemia mice
[0083] Twenty-four healthy male KunMing mice with a body weight of 24-32 g were taken, and after one week of adaptive culture, they were randomly divided into 4 groups, namely the control group, hyperuricemia model group, Lactobacillus reuteri CCFM1132 intervention group and allopurin Alcohol intervention group (allopurinol). Except for the control group, 500mg / kg BW hypoxanthine was intragastrically administered to other groups every day, and 200mg / kg BW oxonate potassium was intraperitoneally injected 1 hour later; 1 hour before oxonate potassium treatment, the control group and the hyperuricemia model group were given 100g / L skimmed milk, Lactobacillus reuteri CCFM1132 intervention group was given 1.0×10 9 CFU / Lactobacillus reuteri CCFM1132, the allopurinol group was given 5mg / kgBW allopurinol. The experimental groups and processing methods are shown in Table 2:
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