A kind of fish demineralized bone matrix and preparation method thereof

Abstract

The invention discloses a fish demineralized bone matrix and a preparation method thereof. The preparation method comprises the following steps: placing fresh fish bones in a reaction kettle, and performing the following treatments in the reaction kettle in sequence: a sodium chloride solution at 4° C. Stir for 8h-SDS solution at 4°C for 8h-pure water wash-glycerol for 8h-pure water wash-hydrogen peroxide solution for 8h at 37°C-pure water wash-anhydrous ether for 8h at 4°C-protease solution 4 Stir at ℃ for 0.5h-pure water washing-dehydrated ethanol dehydration, take out the dehydrated fish bones from the reaction kettle, freeze-dry to obtain fish demineralized bone matrix. The fish demineralized bone matrix prepared by the invention preserves the spatial structure of the fish bone skeleton, has good biocompatibility, biodegradability and bone repair ability, and has no risk of zoonotic disease. The obtained product has good mechanical strength and can be used for various non-load-bearing bone defect repair and load-bearing bone defect auxiliary repair.

Description

technical field [0001] The invention relates to a decalcified bone matrix and a preparation method thereof, in particular to a fish decalcified bone matrix and a preparation method thereof, and belongs to the technical field of bone defect repair materials. Background technique [0002] The number of patients with various types of bone defects caused by trauma, disease, infection, etc. has been high every year, which has brought a lot of inconvenience to the patients themselves and their families. How to repair bone defects and restore corresponding functions in a short period of time is the biggest appeal of patients, and it is also the direction of the efforts of the majority of medical workers. [0003] At present, in clinical practice, autologous bone transplantation has always been regarded as the "gold standard" for bone repair. However, problems such as secondary trauma and insufficient bone mass caused by bone harvesting have affected the wide application of autologo...

AI Technical Summary

Problems solved by technology

[0005] my country is a country with large aquatic products, and there are a huge number of various types of aquatic product processing enterprises. The fish bones left over from the processing of aquatic products by various enterprises are generally discarded as solid waste, or sold to feed production enterprises at a low price for feed production, very low added value

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