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A fast technology with seven leaves and one flower, applied in the field of plant cultivation, can solve the problems of uneven germination and low germination rate.
Active Publication Date: 2022-03-25
CHONGQING NORMAL UNIVERSITY
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Problems solved by technology
Due to the post-ripening phenomenon, the seed embryo of Aesculus aescini needs at least two consecutive winters and one
Method used
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Embodiment 1
[0038] The quick seedling-forming method of Aesculus aesculata of the present embodiment comprises the following steps,
[0039] S1, pretreatment: the mature Aesculus esculenta seeds are cleaned, and the outer testa is removed, and placed in a 1% hydrogen peroxide solution for soaking for 5 minutes.
[0040] S2. Stratification treatment: the pretreated seeds were placed at 20° C. under dark conditions for stratification treatment for 40 days.
[0041] S3. Germination: Plant the stratified seeds in wet perlite for germination. During the germination process, the ambient temperature is kept at 22°C, and the photoperiod is 12h·d -1 , with an illumination intensity of 30 μmol m -2 ·s -1 .
[0042] S4. Transfer the germinated seeds with 3-5 mm long radicles to the seedling-forming medium, and culture them at 22° C. to obtain Aesculus aesculus seedlings.
Embodiment 2
[0044] This embodiment includes most of the operational steps of Embodiment 1, and differs from Embodiment 1 in that:
[0045] In this embodiment, before the layering treatment, in step S1, the seeds of Aesculus aesculus were soaked in a 1% hydrogen peroxide solution for 5 minutes, and then the seeds were placed in a 10 mg / L gibberellin solution Soak in medium, 20°C and dark conditions for 48 hours.
Embodiment 3
[0047] This embodiment includes most of the operational steps of Embodiment 1, and differs from Embodiment 1 in that:
[0048] In this embodiment, before the layering treatment, in step S1, the seeds of Aescinus aesculus were soaked in a 1% hydrogen peroxide solution for 5 minutes, and then the seeds were placed in a 50 mg / L gibberellin solution Soak in medium, 20°C and dark conditions for 48 hours.
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Abstract
The invention discloses a rapid seedling formation method of paris polyphylla, which comprises the following steps: S1, pretreatment: cleaning mature paris polyphylla seeds, removing testa, and soaking in a hydrogen peroxide solution with the concentration of 1% for 5 minutes; s2, stratification treatment: subjecting the pretreated seeds to stratification treatment for 30-40 days under a dark condition at the temperature of 20 DEG C; s3, germination: the seeds subjected to stratification treatment are planted in wet perlite for germination, and in the germination process, the environment temperature is kept at 22 DEG C, the illumination period is 12 h.d <-1 >, and the illumination intensity is 30 [mu] mol.m <-2 >. S <-1 >; s4, the germinated seeds with radicles with the length of 3-5 mm are transferred into a seedling forming substrate and cultured at the temperature of 22 DEG C, and paris polyphylla seedlings are obtained; the high germination rate can be obtained in a short time, the defects of long germination time and low germination rate in the prior art are overcome, and the method has the advantages of being short in germination period and orderly in germination.
Description
technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for rapidly growing seedlings of Aesculus aesculus. Background technique [0002] Aescin (Parispolyphylla var.chinensis (Franchet) H.Hara) is a plant of the genus Chonglou in the Liliaceae (Liliaceae). It is a kind of precious Chinese medicinal material, also known as Huazhonglou. The advantages of using seeds to propagate seedlings are that the reproduction coefficient is high, the root system of the seedlings is complete, and the growth is robust. Therefore, seed propagation is always a focus of research as one of the main methods of Aesculus esculenta seedling breeding. But, the embryo of Echinacea aescine seed is still in the globular embryo stage just after detaching from the mother plant, and then in the post-ripening process, the embryo goes through the stages of oval embryo, cylindrical embryo, heart-shaped embryo and embryo embryo, only when the embry...
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