Lactobacillus acidophilus capable of relieving ulcerative colitis and application of lactobacillus acidophilus

A technology for ulcerative colitis and Lactobacillus acidophilus, applied in the field of microorganisms, can solve the problems of many limitations of side effects of inflammatory bowel disease, and achieve the effects of increasing colon length, low cost, and easy industrial production.

Active Publication Date: 2022-03-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these three drug treatments have many side effects and limitations in the treatment of inflammatory bowel disease.

Method used

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  • Lactobacillus acidophilus capable of relieving ulcerative colitis and application of lactobacillus acidophilus
  • Lactobacillus acidophilus capable of relieving ulcerative colitis and application of lactobacillus acidophilus
  • Lactobacillus acidophilus capable of relieving ulcerative colitis and application of lactobacillus acidophilus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1: Screening and strain identification of Lactobacillus acidophilus CCFM1200

[0058] 1. Screening

[0059] With healthy human feces samples from Wuhu City, Anhui Province, 0.5g of samples preserved in 30% (v / v) glycerol was added to a 10mL centrifuge tube containing 4.5mL of normal saline under a sterile environment to obtain 10 -1 Diluent, repeat the above dilution steps to obtain 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Dilution: draw 100 μL of gradient dilutions of different gradients and spread them on the MRS solid medium, and incubate at 37°C for 72 hours to obtain a diluted coating plate; pick typical colonies on the diluted coating plate and mark them on the MRS solid medium. Line, cultivated at 37°C for 48 hours to obtain purified colonies; pick the purified colonies and inoculate them into MRS liquid medium, and cultivate at 37°C for 48 hours to obtain strain CCFM1200.

[0060] 2. Identification

[0061] The genome of CCFM1200 was extracted, the 16S r...

Embodiment 2

[0062] Embodiment 2: the preparation of Lactobacillus acidophilus bacteria suspension

[0063] The preparation method of Lactobacillus acidophilus NCFM or Lactobacillus acidophilus CCFM1200 bacterial liquid is as follows:

[0064] Dip the liquid of Lactobacillus acidophilus and streak on the MRS solid medium, culture at 37°C for 48 hours to obtain a single colony;

[0065] Pick a single colony and insert it into the MRS liquid medium, and cultivate it at 37°C for 24h to obtain an activation solution; insert the activation solution into the MRS liquid medium according to the inoculation amount of 1% (v / v), and cultivate it at 37°C for 24h, Obtain the primary seed liquid;

[0066] The primary seed solution was inserted into the MRS liquid medium according to the inoculation amount of 1% (v / v), and cultured at 37° C. for 24 hours to obtain the secondary seed solution;

[0067] Put the secondary seed liquid into the MRS liquid medium according to the inoculum amount of 1% (v / v),...

Embodiment 3

[0068] Example 3: Effects of Lactobacillus acidophilus CCFM1200 on the body weight and colon length of mice with ulcerative colitis

[0069] Take 32 male pathogen-free (SPF) C57BL / 6J mice aged 6-8 weeks, and raise them under the conditions of room temperature 22-24°C, humidity 40-60%, 12h / 12h day and night, free access to food and water After feeding for 1 week, they were randomly divided into 4 groups with 8 rats in each group. The 4 groups were: control group, model group, Lactobacillus acidophilus (Lactobacillus acidophilus) NCFM group, Lactobacillus acidophilus (Lactobacillus acidophilus) ) CCFM1200 group.

[0070] The experiment started after one week of adaptive feeding of the animals, and the experiment lasted for 2 weeks. The specific treatment is as follows:

[0071] Control group: During the whole experimental period, the mice drank water normally and fed 200 μL of normal saline as a control;

[0072] Modeling group: drink water normally from the 1st to 7th day, d...

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Abstract

The invention discloses lactobacillus acidophilus capable of relieving ulcerative colitis and application of the lactobacillus acidophilus, and belongs to the technical field of microorganisms. According to the invention, a strain of lactobacillus acidophilus CCFM1200 is screened, and the lactobacillus acidophilus CCFM1200 has the effect of relieving ulcerative colitis caused by DSS, which is specifically reflected as follows: (1) the weight loss of mice with ulcerative colitis is reduced; (2) the colon length of a mouse with ulcerative colitis is obviously increased; (3) the immune factor level in the colons of the mice with ulcerative colitis is obviously improved; (4) the expression quantity of cell chemokine receptors in colons of mice with ulcerative colitis is obviously reduced; (5) the content of short-chain fatty acid in excrement of mice with ulcerative colitis is obviously increased; and (6) regulating intestinal flora of the mouse with ulcerative colitis. The lactobacillus acidophilus CCFM1200 disclosed by the invention has a huge application prospect in the aspect of preparing food or medicines for preventing and / or treating ulcerative colitis.

Description

technical field [0001] The invention relates to a strain of Lactobacillus acidophilus that can relieve ulcerative colitis and its application, especially a strain that can relieve ulcerative colitis, which can be added to various health foods and health foods, and belongs to the technical field of microorganisms . Background technique [0002] Inflammatory bowel disease (IBD), mainly including Crohn's disease (CD) and ulcerative colitis, is a chronic intestinal inflammation characterized by impaired intestinal epithelial barrier function and intestinal immune dysregulation. When the integrity of the intestinal epithelium is compromised, intestinal bacteria and their products penetrate the intestinal barrier, leading to abnormal immune responses and inflammation. In recent years, the number of IBD patients in my country has shown a rapid upward trend. Currently, the etiology of inflammatory bowel disease is unclear. Drugs commonly used in the treatment of inflammatory bowe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P1/00A61P29/00A23L33/135C12R1/23
CPCA61K35/747A61P1/00A61P29/00A23L33/135A23V2002/00A23V2400/113A23V2200/3204Y02A50/30
Inventor 杨波陈卫黄铮陈海琴刘小鸣崔树茂陆文伟赵建新张灏
Owner JIANGNAN UNIV
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