Circulating tumor cell separating and capturing device and method thereof

A technology for capturing devices and tumor cells, applied in cell dissociation methods, tumor/cancer cells, animal cells, etc., can solve the problems of reduced specificity and efficiency, and achieve the effects of low cost, stable performance and good reliability

Pending Publication Date: 2022-03-29
ZIGZAG BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since 30% of tumor cells from different tissue sources do not express EpCAM, and EpCAM is lost due to the possible EMT of tumor ce

Method used

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  • Circulating tumor cell separating and capturing device and method thereof
  • Circulating tumor cell separating and capturing device and method thereof
  • Circulating tumor cell separating and capturing device and method thereof

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Embodiment Construction

[0022] Below in conjunction with accompanying drawing, the present invention is further explained and described.

[0023] A circulating tumor cell separation and capture device, the separation device includes a microfluidic chip (1), and the microfluidic chip (1) is sealed and connected with a sample tube (21), a first waste liquid collection tube ( 22), solution pipe (23), second waste liquid collection pipe (24) and enrichment liquid collection pipe (25), microfluidic chip (1) and sample pipe (21), first waste liquid collection pipe (22 ), the solution pipe (23), the second waste liquid collection pipe (24) and the enrichment liquid collection pipe (25) are all detachable assembly methods, which are convenient for replacement when different samples are separated; generally speaking, microfluidic The chip (1) is disposable, and the various tubes used above are preferably centrifuge tubes; the sample tube (21) is used to provide the sample to be separated to the microfluidic c...

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Abstract

The invention relates to a circulating tumor cell (CTC) separating and capturing device, in particular to a circulating tumor cell (CTC) separating and capturing device which is simple in structure, stable and reliable and comprises a micro-fluidic chip. The micro-fluidic chip is hermetically connected with a sample tube, a first waste liquid collecting tube, a solution tube, a second waste liquid collecting tube and an enriched liquid collecting tube; the sample tube, the first waste liquid collecting tube, the solution tube, the second waste liquid collecting tube and the enriched liquid collecting tube are respectively connected with a pneumatic driving device. The invention has the advantages of one-step separation, short time, low cost, integrated microfluidic chip, automatic operation flow, simple structure, stable performance and good reliability. After a blood sample is separated by the device, a suspension containing circulating tumor cells (CTC) is collected in the enrichment liquid collecting tube.

Description

technical field [0001] The invention relates to a circulating tumor cell (CTC) separation and capture device, which drives blood samples into a microfluidic chip through air pressure control, enabling the sample to separate and capture circulating tumor cells (CTC) in the microfluidic chip. Background technique [0002] At present, the continuous increase of tumor morbidity and mortality has become one of the serious problems in today's society. At present, the discovery and diagnosis of most tumors still rely on imaging examinations, traditional tumor markers and tissue biopsy. Traditional invasive tissue biopsy requires removing a tissue or cell sample from the patient, a procedure that often brings certain risks and pain to the patient. More importantly, tissue biopsy is not suitable for repeated extraction and is prone to complications, and it has the risk of spreading cancer cells. Related studies have confirmed that in the early stages of cancer, tumors release circu...

Claims

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Application Information

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IPC IPC(8): C12M1/24C12M1/00C12N5/09
CPCC12M47/04C12N5/0693C12N2509/00
Inventor 刘宗彬
Owner ZIGZAG BIOTECH LTD
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