Application of radix ampelopsis extract in preparation of medicine for preventing and/or treating polycystic ovarian syndrome

A polycystic ovary and extract technology, applied in the field of drug preparation, can solve the problems of preventing and treating polycystic ovary syndrome, etc., and achieve the effects of improving ovarian pathology, increasing body weight, and increasing uterine weight

Pending Publication Date: 2022-04-01
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Baijiao is a commonly used traditional Chinese medicine, which is widely used clinically, but it has not been used to prevent and treat polycystic ovary syndrome.

Method used

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  • Application of radix ampelopsis extract in preparation of medicine for preventing and/or treating polycystic ovarian syndrome
  • Application of radix ampelopsis extract in preparation of medicine for preventing and/or treating polycystic ovarian syndrome
  • Application of radix ampelopsis extract in preparation of medicine for preventing and/or treating polycystic ovarian syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: the preparation of Radix Sinensis Extract

[0037] Take an appropriate amount of white pomegranate medicinal material, decoct twice with 10 times the amount and 8 times the amount of water successively, filter with gauze, and combine the two filtrates. The aqueous extract of Potassium officinalis was concentrated to 0.3 g / ml using a rotary evaporator.

Embodiment 2

[0038] Embodiment 2: Construction polycystic ovary syndrome (PCOS) rat model

[0039] Rats with normal estrous cycle were selected from female SD rats and divided into normal group and model group. Rats in the normal group were administered with 1% sodium carboxymethylcellulose 4ml / kg.d, and rats in the model group were administered with letrozole 1mg / kg+1% sodium carboxymethylcellulose 4ml / kg. d, 21 days in a row. Vaginal smears were made on the rats on the 10th day after modeling. The vaginal smears of the rats were observed under an optical microscope for 10 consecutive days. It was observed that white blood cells persisted in the vaginal epithelium of rats. If the estrous cycle was lost, the modeling was successful; otherwise, the modeling failed and should be rejected.

experiment example

[0041] The modeled rats were randomly divided into PCOS group and Baishui group (BL), and a normal group (Normal) was set. The Potassium officinalis group was given the Potassium officinalis extract at a dosage of 2g / kg, and the PCOS group and the normal group were given the same volume of normal saline, and were gavaged continuously for 14 days.

[0042] After the administration, the rats were anesthetized, and 4-5 ml of blood was collected from the abdominal aorta, centrifuged at 4000 r / min for 15 min, and the supernatant was collected for the determination of the levels of LH, FSH and T in the serum. Serum was stored frozen at -80°C. Two ovaries were taken, one ovary was fixed with 4% paraformaldehyde for histopathological analysis, and one side was frozen at -80°C.

[0043]In the present invention, the experimental process from PCOS modeling to the intervention of the Radix Cortex Potassica Extract, and the collection of materials (serum, bilateral ovaries) is as follows:...

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Abstract

The invention discloses application of a radix ampelopsis extract in preparation of a medicine for preventing and/or treating polycystic ovarian syndrome. The polyovarian syndrome (PCOS) is an endocrine metabolic disease, and the clinical main manifestations are menorrhea or amenorrhea, hyperandrogenesis, polycystic ovarian alteration and the like. On the human body, menstruation can show the ovulation condition of a woman; on the rat body, the ovulation condition of the rat is displayed by the mood cycle. Rats are taken as experimental subjects, a PCOS group, an ampelopsis japonica group and a normal group are set, and the influence of the ampelopsis japonica extract on the PCOS rat physical state is researched by representing the estrus cycle, the ovary state, the sex hormone level, the body mass, the ovary weight, the uterus weight and the like of the rats.

Description

technical field [0001] The invention belongs to the field of medicine preparation, and in particular relates to the application of the extract of Radix Pachyrhiza in the preparation of medicines for preventing and / or treating polycystic ovary syndrome. Background technique [0002] Polycystic ovary syndrome is a disease of endocrine and metabolic disorders. The main clinical manifestations are oligomenorrhea or amenorrhea, hyperandrogenism, and polycystic changes in the ovary. There is no term polycystic ovary syndrome in the ancient books of traditional Chinese medicine, but according to its clinical manifestations, it can be classified into the categories of "late menstruation", "amenorrhea", "infertility" and "uterine bleeding" in the field of traditional Chinese medicine. In the theory of traditional Chinese medicine, the liver stores blood and governs catharsis, sexual happiness, evil and depression. If there is excess qi, blood deficiency, natural depression, or irrita...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/87A61P15/08A61P5/24A61P15/00A61K125/00
Inventor 汤庆发朱惠卿巫园园庄子铭梁泳琳陈飞龙
Owner SOUTHERN MEDICAL UNIVERSITY
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