Microecological preparation for purifying aquaculture tail water
A micro-ecological preparation, aquaculture technology, applied in biological water / sewage treatment, water / sludge / sewage treatment, chemical instruments and methods, etc. , to achieve the effect of improving purification of aquaculture wastewater, better adaptability and effect, and improving water quality
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Embodiment 1
[0032] (1) Preparation of Acetobacter agent, take a ring of Acetobacterfabarum (Acetobacterfabarum, preservation number CICC No. 10883) from the inclined surface of the test tube, inoculate it into 30-100mL sterilized acetic acid bacteria medium, and incubate at 30-32 ℃, 100-125r·min -1 Cultivate on a shaker for 22-26h, then insert it into 0.6-0.8L sterilized acetic acid bacteria culture medium, at 30-32°C, 100-125r min -1 Cultivate on a shaker for 22-26 hours, and make a seed solution of the bacteria for use. At this time, the number of viable bacteria in the solution is ≥108CFU·mL -1 .
[0033] Acetobacter lovaniensis (Acetobacter lovaniensis, preservation number is ATCC No.12875) is cultivated corresponding strain seed liquid with reference to above-mentioned culture method;
[0034] Two kinds of Acetobacter are formulated into Acetobacter agent in a ratio of 1:1;
[0035] The above-mentioned acetic acid bacteria culture medium is, glucose 98g L -1 , yeast extract 9g·L ...
Embodiment 2
[0045] See Example 1 for the configuration of the seed liquid of each bacterial strain in the EM bacterial agent in this embodiment, the difference is that the ratio of each raw material is different, and the preparation method is the same;
[0046] This embodiment provides a kind of micro-ecological preparation for the purification of aquaculture tail water, which is prepared from the following raw materials in parts by weight: 60 parts of EM bacterial agent, 80 parts of nutrient agent;
[0047] Wherein, the EM bacterial agent is composed of the following bacterial agents in parts by weight: 40 parts of Acetobacter agent, 15 parts of Bacillus agent, and 10 parts of nitrifying bacteria agent;
[0048] The nutritional agent is prepared from the following raw materials in parts by weight: 10 parts of glucose, 3 parts of brown sugar and 3 parts of soybean meal.
[0049] The preparation method is to mix the seed liquids of each bacterial agent in the above ratio to make EM microbi...
Embodiment 3
[0051] See Example 1 for the configuration of the seed liquid of each bacterial strain in the EM bacterial agent in this embodiment, the difference is that the ratio of each raw material is different, and the preparation method is the same;
[0052] This example provides a microecological preparation for the purification of aquaculture tail water, which is prepared from the following raw materials in parts by weight: 80 parts of EM bacterial agent, 100 parts of nutritional agent;
[0053] Wherein, the EM microbial agent is composed of the following bacterial agents in parts by weight: 40 parts of Acetobacter agent, 15 parts of Bacillus agent, and 20 parts of nitrifying bacteria agent;
[0054] The nutrient is prepared from the following raw materials in parts by weight: 15 parts of glucose, 5 parts of brown sugar and 5 parts of soybean meal.
[0055] The preparation method is to mix the seed liquids of each bacterial agent in the above ratio to make EM microbial agent, and the...
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