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Detection method for mycotoxin in spices and products thereof

A technology for mycotoxins and a detection method, which is applied in the field of detection of mycotoxins in spices and their products, can solve the problems of time-consuming and laborious, inability to detect mycotoxins at the same time, etc., and achieve the effects of improving purification effect, saving manpower and material resources, and improving efficiency.

Pending Publication Date: 2022-04-05
CHONGQING INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The detection of mycotoxins in spices and their products mainly includes high performance liquid chromatography, high performance liquid chromatography tandem mass spectrometry / mass spectrometry and enzyme-linked immunosorbent assay, but most of these methods are only for a single mycotoxin in spices and their products It is impossible to detect more than ten kinds of mycotoxins at the same time, so it is time-consuming and laborious

Method used

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  • Detection method for mycotoxin in spices and products thereof
  • Detection method for mycotoxin in spices and products thereof
  • Detection method for mycotoxin in spices and products thereof

Examples

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Embodiment 1

[0063] This embodiment provides a detection method for mycotoxins in spices and their products, including the following process:

[0064] 1. Optimization of mass spectrometry conditions: the preparation concentrations were aflatoxin B1 50 μg / L, aflatoxin B2 100 μg / L, aflatoxin G1 100 μg / L, aflatoxin G2 100 μg / L, ochratoxin A 100 μg / L, fuma Toxin B1 2000μg / L, fumonisin B2 2000μg / L, deoxynivalenol 1000μg / L, 3-acetyldeoxynivalenol 1000μg / L, 15-acetyldeoxynivalenol 1000μg / L, T-2 toxin 500μg / L, HT-2 toxin 500μg / L, zearalenone 100μg / L, variegated aspergillus toxin 100μg / L mixed standard solution, diluted to a certain concentration of standard solution, with 10μL / min flow rate into the mass spectrometer. In the multiple reaction monitoring (MRM) mode, the standard substance is firstly analyzed by mass spectrometry (Q1 scan) to obtain the molecular ion peak, and the corresponding parent ion peak is selected, and the product ion is subjected to secondary mass spectrometry analysis (...

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Abstract

The invention belongs to the field of food detection methods, and discloses a method for detecting fungaltoxin in spices and products thereof. A liquid chromatogram-tandem mass spectrum / mass spectrum determination method is used for determining the content of aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A, fumonisins B1, fumonisins B2, deoxynivalenol, fumonisins B1, fumonisins B2, deoxynivalenol, fumonisins B1, fumonisins B2, fumonisins B1, fumonisins B2, fumonisins B1, fumonisins B2, fumonisins B1, fumonisins B1, fumonisins B2, fumonisins B1, fumonisins B2, fumonisins B1, fumonisins The medicine is prepared from the following raw materials: 3-acetyl deoxynivalenol, 3-acetyl deoxynivalenol, 15-acetyl deoxynivalenol, T-2 toxin, HT-2 toxin, zearalenone and sterigmatocystin. By setting the mass spectrum condition and liquid phase condition of each mycotoxin, various mycotoxins in spices and products thereof can be effectively extracted, purified, separated and detected, the experiment precision and detection limit both meet the experiment requirements, and simultaneous detection of 14 mycotoxins is realized.

Description

technical field [0001] The invention belongs to the field of food detection methods, and in particular relates to a detection method of mycotoxins in spices and their products. Background technique [0002] Mycotoxins are metabolites produced by fungi growing in food or feed that are harmful to humans and animals. Common mycotoxins are aflatoxins. In 1960, the "Turkey X Disease" that caused the death of more than 100,000 turkeys in the UK was caused by aflatoxin in the feed. The main symptoms are loss of appetite, drooping wings, lethargy and death after being infected, with the head and feet stretched back when dead. Dissection showed hepatic hemorrhage, necrosis, and renal enlargement. Some bleeding syndromes are also caused by mycotoxins. For example, the T2 toxin produced by Fusarium pseudomycoides and Fusarium pirosporum has acute symptoms of general convulsions and death from heart failure; subacute or chronic poisoning often manifests as gastritis, nausea, oral ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCY02A50/30
Inventor 黄思瑜董宪兵邓宇杰李红侯美玲
Owner CHONGQING INST FOR FOOD & DRUG CONTROL
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