Micro-fluidic chip adaptable to fluorescent quantitative PCR (Polymerase Chain Reaction) instrument
A microfluidic chip and fluorescence quantitative technology, which is applied in specific-purpose bioreactors/fermenters, biochemical instruments, laboratory containers, etc., can solve the problems of uneven sample addition and cumbersome process. Achieve the effect of improving the use effect and solving the uneven sample addition
Pending Publication Date: 2022-04-12
无锡博奥玛雅医学科技有限公司 +1
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[0005] In view of the above problems, the present invention proposes a microfluidic chip that can be adapted to a fluorescent quantitative PCR instrument, which is used to solve the problems of uneven sample addition and cumbersome procedures in the prior art when testing large batches of samples
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[0032] In order to clearly illustrate the technical characteristics of this patent, the following describes this patent in detail through specific implementation methods and in conjunction with the accompanying drawings.
[0033] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "longitudinal", "lateral", "horizontal" , "Inner", "Outer", "Front", "Back", "Top", "Bottom" and other indicated orientations or positional relationships are based on the orientations or positional relationships shown in the attached drawings, or when the inventive product is used Orientation or positional relationship that is conventionally placed is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot b...
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The invention discloses a micro-fluidic chip adaptable to a fluorescent quantitative PCR instrument, belongs to the technical field of molecular diagnosis, and is used for solving the problems of non-uniform sample adding of a sample adding solution and tedious flow during large-batch sample testing in the prior art. The micro-fluidic chip comprises a chip main body with a plurality of visual reaction tanks, the shape of the chip main body is matched with that of a PCR instrument, at least one bent micro-fluidic channel is arranged in the chip main body, and the lower convex parts of the micro-fluidic channels are communicated with the reaction tanks. The capillary bending area at the upper convex part of the micro-fluidic channel can prevent liquid in the micro-fluidic channel from flowing due to centrifugal acceleration during centrifugal liquid separation, so that sample adding liquid flowing into the reaction tank is relatively uniform, the problem of non-uniform sample adding of the sample adding liquid is solved, and the use effect is improved.
Description
technical field [0001] The invention relates to the technical field of molecular diagnosis, in particular to a microfluidic chip adaptable to a fluorescent quantitative PCR instrument. Background technique [0002] Fluorescent quantitative PCR technology is a very common quantitative detection technology in the field of molecular diagnosis at present. By mixing reagents and samples in an 8-row, 96-well plate, and then placing them in a dedicated fluorescent quantitative PCR instrument for temperature-controlled reaction, the As the reaction progresses, a specific fluorescent signal will be generated, and the instrument can achieve the function of diagnosis after collecting the signal. [0003] Common detection indicators may involve multiple, and one detection sample needs to be repeatedly added to multiple reaction units. At present, common reaction containers that can match fluorescent quantitative PCR instruments are mainly PCR tubes, eight-row strips, and 96-well plates....
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Login to View More IPC IPC(8): B01L3/00C12M1/00C12M1/38C12M1/34
Inventor 李东
Owner 无锡博奥玛雅医学科技有限公司