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Model construction of influence of endoplasmic reticulum stress on NLRP3 on myocardial ischemia

A technique for endoplasmic reticulum stress and myocardial ischemia, which can be used in preparations, material testing products, measuring devices and other directions for in vivo experiments, and can solve problems such as unclear mechanism of action

Pending Publication Date: 2022-04-29
川北医学院附属医院
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Problems solved by technology

[0003] In recent years, the role of pyroptosis in ischemia-reperfusion injury has attracted much attention, but its mechanism is unclear

Method used

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  • Model construction of influence of endoplasmic reticulum stress on NLRP3 on myocardial ischemia
  • Model construction of influence of endoplasmic reticulum stress on NLRP3 on myocardial ischemia
  • Model construction of influence of endoplasmic reticulum stress on NLRP3 on myocardial ischemia

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Embodiment Construction

[0039] The following is further described in detail through specific implementation methods:

[0040] The embodiment is basically as attached figure 1 Shown: the effect of endoplasmic reticulum stress on NLRP3 in the construction of a model of myocardial ischemia The specific implementation process is as follows: the effect of endoplasmic reticulum stress on NLRP3 in the construction of a model of myocardial ischemia, using ligation / release of the left coronary artery in mice The descending artery and hypoxia and reoxygenation of cardiomyocytes were used to establish in vivo and in vitro models of myocardial ischemia-reperfusion injury, and then the content of calpain was adjusted during the experiment to change the stability of endoplasmic reticulum stress. The myocardial ischemia model of the changing process was continuously compared with the myocardial infarct size and the expression level of NLRP3 protein to realize the demonstration effect of the model.

[0041] (1) Imp...

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Abstract

The invention discloses construction of a model of influence of endoplasmic reticulum stress on NLRP3 in myocardial ischemia in the field of medical model construction so as to clarify the effect of pyroptosis in ischemia reperfusion injury and the action mechanism of the pyroptosis, and according to the technical scheme, an in-vivo model of myocardial ischemia / reperfusion injury of a mouse is established by adopting a method of ligation / release of left anterior descending coronary artery; a myocardial ischemia / reperfusion injury in-vitro model is constructed by carrying out oxygen deficit and reoxygenation on cultured myocardial cells, and then endoplasmic reticulum stress is regulated and controlled by intervening calpain in an experiment process. Finally, by comparing the myocardial infarction area and the NLRP3 protein expression level, the effect of calpain in regulating endoplasmic reticulum stress-mediated pyroptosis in ischemic myocardial injury is determined. According to the technical scheme, a myocardial ischemia reperfusion model is mainly used for verifying existence of pyroptosis in myocardial ischemia and the effect of endoplasmic reticulum stress in the myocardial ischemia model.

Description

technical field [0001] The invention belongs to the field of medical model construction, in particular to the construction of a model of myocardial ischemia in which endoplasmic reticulum stress affects NLRP3. Background technique [0002] For patients with myocardial infarction, early and effective restoration of ischemic myocardial blood supply is the fundamental measure to prevent the development of ischemic myocardial injury, reduce the infarct size and improve the clinical prognosis. However, myocardial ischemia-reperfusion injury has become a problem that restricts ischemic myocardium from benefiting from reperfusion therapy. The mechanism of myocardial ischemia-reperfusion injury has been widely and deeply studied at home and abroad, but the specific mechanism is still not very clear. Therefore, further research on the occurrence and development mechanism of ischemia-reperfusion injury has important clinical significance for its prevention. [0003] In recent years,...

Claims

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Application Information

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IPC IPC(8): A61K49/00G01N33/573G01N33/68
CPCA61K49/0004G01N33/6887G01N33/573G01N2800/324
Inventor 岳荣川胡厚祥郑在勇王小波罗瑜吕明明赵雪梅兰美德谭欣刘延旭罗豪文聪
Owner 川北医学院附属医院
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