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Application of lotus corniculatus seed extract and rhamnosyluridine

A rhamnose-based, lavender root technology, applied in cosmetic preparations, skin care preparations, pharmaceutical formulations, etc., can solve problems such as the effect of lavender seed extract that has not been reported, and reduce skin oil. Secretion, reducing the effect of astringent pores

Pending Publication Date: 2022-05-06
SHANGHAI JAKA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no report on the effect of japonicus japonicus seed extract on skin oil control and astringent pores

Method used

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  • Application of lotus corniculatus seed extract and rhamnosyluridine
  • Application of lotus corniculatus seed extract and rhamnosyluridine
  • Application of lotus corniculatus seed extract and rhamnosyluridine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052]This embodiment provides a method for extracting and separating rhamnosyluridine, the specific steps are as follows:

[0053] 1) Weigh 500 g of japonicus japonicus seeds, use an oil press to squeeze, and collect the filter cake.

[0054] 2) Add water 10 times the weight of the filter cake, extract at 80°C for 2 hours, and filter the obtained extract with 10 μm and 5 μm polypropylene membranes in turn to obtain a filtrate.

[0055] 3) Adsorb the filtrate with a D101 macroporous resin column, wash with pure water until the effluent is basically colorless, then wash with 30% ethanol until the effluent is basically colorless, collect the 30% ethanol eluate, and concentrate to the ethanol content 4% (here the ethanol concentration is the measured value of the alcohol meter).

[0056] 4) Adsorb the concentrated solution with an MCI CHP20P resin column, and wash the column with water, 10% ethanol, 20% ethanol, and 30% ethanol in sequence. Rhamnosyluridine is mainly concentrat...

Embodiment 2

[0059] This example provides a method for extracting and separating rhamnosyluridine. The specific steps are basically the same as in Example 1, except that the collected 30% ethanol eluate is not concentrated, and the 30% The ethanol eluate was adsorbed with MCICHP20P resin.

[0060] Specific steps are as follows:

[0061] 1) 500 g of japonicus japonicus seeds are pressed by an oil press to collect the filter cake.

[0062] 2) Add water 10 times the weight of the filter cake, extract at 80°C for 2 hours, and filter the obtained extract with 10 μm and 5 μm polypropylene membranes in turn to obtain a filtrate.

[0063] 3) Adsorb the filtrate with a D101 macroporous resin column, wash with pure water until the effluent is basically colorless, then wash with 30% ethanol until the effluent is basically colorless, and collect the 30% ethanol eluate.

[0064] 4) Adsorb the 30% ethanol eluate with the MCI CHP20P resin column, and wash the column with water, 10% ethanol, and 20% eth...

Embodiment 3

[0079] SEB-E6E7 cell oil secretion test:

[0080] The japonicus japonicus seed extract prepared by the same method as in the above-mentioned example 1, and the rhamnosyluridine separated by the same method as in the embodiment 1 were respectively prepared into an aqueous solution with a mass fraction of 0.2%, which were respectively marked as test substance 1 (Jiamen japonicus L. root seed extract); test substance 2 (rhamnosyluridine).

[0081] 1) Cell model construction and testing methods

[0082] SEB-E6E7 cells (purchased from Shanghai Baili Biotechnology Co., Ltd.) were used to construct a lipid model with 0.00625% linoleic acid. Add 100 μL complete medium and 100 μL linoleic acid to each well of model group, add 50 μL linoleic acid, 50 μL test substance 1 and 100 μL medium to each well of sample group 1, add 50 μL linoleic acid and 50 μL test substance 2 to each well of sample group 2 and 100 μL medium. Three parallel experiments were set up for each group. 37°C, 5% C...

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Abstract

The invention discloses application of a lotus corniculatus seed extract and rhamnosyl uridine, and particularly relates to application of the lotus corniculatus seed extract and the rhamnosyl uridine in preparation of products for controlling oil and / or minimizing pores. It is verified for the first time that both the lotus corniculatus seed extract and the rhamnosyluridine separated from the lotus corniculatus seed extract have the effects of reducing skin grease secretion and shrinking pores, and the lotus corniculatus seed extract and the rhamnosyluridine can be widely applied to personal care products and have very good application prospects.

Description

technical field [0001] The present invention relates to the application technical field of compounds, and relates to the use of japonicus japonicus seed extract and rhamnosyl uridine, in particular to the use of japonicus japonicus seed extract and rhamnosyl uridine in the preparation of oil control and / or use in pore tightening products. Background technique [0002] The full name of rhamnosyluridine is 5'-O-α-L-rhamnosyluridine, which belongs to nucleoside components, and its chemical structure is shown below. [0003] [0004] At present, there are only reports on its chemical synthesis. Lluis Ballell et al. reported the synthesis method of this compound in 2005, and confirmed that it is an inhibitor of RmlB protein (dTDP-α-D-glucose-4,6-dehydratase), which can effectively treat tuberculosis. [0005] Lotus corniculatus (Lotus corniculatus) is a plant of the genus Lotus (Leguminosae), also known as five-leaf clover, ox horn flower, and bird's distance grass. In my c...

Claims

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Application Information

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IPC IPC(8): A61K8/60A61K8/9789A61Q19/00A61Q19/10
CPCA61K8/606A61K8/9789A61Q19/008A61Q19/10Y02A50/30
Inventor 程璐孔令珊周利丹卢伊娜熊玥张树义田军
Owner SHANGHAI JAKA BIOTECH CO LTD
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