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Flow type door closing method and application

A flow and antibody technology, applied in the fields of instruments, measuring devices, particle and sedimentation analysis, etc., can solve the problems of inaccurate distinction, result influence, lymphocyte loss, etc., to achieve accurate gate method, ensure consistency, and gate Accurate results

Active Publication Date: 2022-05-06
杭州翔宇医学检验实验室有限公司
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Problems solved by technology

The disadvantage of this gate method is that the cells circled in the gate are lymphocytes, and the purity of lymphocytes in the gate is different due to the different experience of the inspectors.
Especially when the abundance of lymphocytes is low, it will cause the loss of lymphocytes and affect the results
Moreover, the gating method cannot accurately distinguish neutrophils, monocytes, and lymphocytes

Method used

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  • Flow type door closing method and application
  • Flow type door closing method and application
  • Flow type door closing method and application

Examples

Experimental program
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Effect test

Embodiment 1 3

[0112] Example 1 Triple Antibody (CD64+CD14+CD45) - Incubate Peripheral Blood Samples with CD64+CD14+CD45 Monoclonal Fluorescent Antibody

[0113] The general diagram of the previous lateral circle, the result is as follows Figure 16 .

[0114] Take the CD45 fluorescent channel to circle the general picture, the result is as follows Figures 17-18 , granulocytes and lymphocytes can be circled.

[0115] With CD14 fluorescent channel in Figure 17 Monocytes are circled among the circled white blood cells, such as Figure 19 shown.

[0116] by Figure 18 granulocytes and lymphocytes, Figure 19 Mononuclear cell gate, get an average value through the fluorescence intensity peak diagram expressed by fluorescent antibody-labeled CD64 antibody on lymph, granulocyte, and monocyte, such as Figure 20 .

[0117] It can be seen from the overall picture of the three antibodies that the three-antibody picture combines all the advantages of the monoclonal antibody and the double an...

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Abstract

The invention discloses a flow type circling method which comprises the following steps: detecting a blood sample co-incubated by a CD45 antibody, a CD14 antibody and a CD64 antibody by using lateral scattered light and CD45 fluorescence, and circling white blood cells in the sample; respectively circling portal neutrophils and lymphocytes in the circled leukocytes by using CD45 fluorescence; in the white blood cells of the enclosure, CD14 fluorescent enclosure mononuclear cells are used. The invention also discloses application of the CD45 antibody and a mixture of the CD14 antibody and the CD64 antibody in preparation of infectious disease diagnosis products, wherein high expression of the CD64 in neutrophile granulocytes is used as an indication of the disease. The flow type door circling method does not excessively depend on experience of technicians, detection consistency is guaranteed, and the door circling mode is more accurate, simpler and clearer. After lymphocytes, monocytes and neutrophils are accurately circled out respectively, whether infectious diseases exist or not can be diagnosed according to the expression of CD64 on the neutrophils.

Description

technical field [0001] The invention relates to the technical field of cell flow detection, in particular to a flow gate method and its application. Background technique [0002] At present, the most common way of gating lymphocytes in blood is: use the forward light FSC and side light SSC of the flow cytometer to do the gating, and the technicians can roughly divide the sample cells into neutrophils and monocytes according to experience. and lymphocytes. The experience of the technicians determines the quality of the ring gate. The disadvantage of this gate method is that the cells circled in the gate are lymphocytes, and the purity of the lymphocytes in the gate varies due to the different experience of the inspectors. Especially when the abundance of lymphocytes is low, it will cause the loss of lymphocytes and affect the results. Moreover, this gating method cannot accurately distinguish neutrophils, monocytes, and lymphocytes. Contents of the invention [0003] Ba...

Claims

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Application Information

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IPC IPC(8): G01N15/14
CPCG01N15/1434G01N2015/144G01N15/149
Inventor 范盘生严倩蓉李正义张涛陆剑
Owner 杭州翔宇医学检验实验室有限公司
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