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Rapid propagation method and culture medium for Mongolian orchid

A technology for multiplying culture medium and culture medium, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as scarcity, and achieve the effects of good seedling quality, high seed germination rate, and fast seedling formation.

Pending Publication Date: 2022-05-13
SHANGHAI CHENSHAN BOTANICAL GARDEN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the scarcity of wild material resources in Mengzilan, research on its sterile sowing and rapid propagation is blank.

Method used

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  • Rapid propagation method and culture medium for Mongolian orchid
  • Rapid propagation method and culture medium for Mongolian orchid
  • Rapid propagation method and culture medium for Mongolian orchid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0033] S1 preparation materials: select healthy Mengzilan plants, artificially pollinate them when they bloom, and use the seeds in the uncracked fruits that develop after pollination as sowing, and the number of days after pollination is the maturity of the seeds.

[0034] S2 Aseptic sowing: Rinse the Mengzilan capsules under running water for 20 minutes, soak them in 75% alcohol for 30 seconds on an ultra-clean workbench, then sterilize them with a 0.1% mercuric chloride solution containing a little Tween 20 for 12 minutes, and rinse them with sterile water for 4 minutes. The second time, the filter paper blotted the water on the surface of the capsules. The capsules were then cut open and the seeds were inoculated on the prepared germination medium.

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Abstract

The invention relates to the field of orchid rapid propagation, and discloses a Mongolian orchid rapid propagation method and a culture medium, and the method comprises the following steps: S1, material preparation, S2, aseptic sowing, S3, clumpy bud proliferation, S4, rooting and seedling strengthening, and S5, transplanting. According to the method, the Mongolian orchid seeds are used as explants, and a large number of seedlings can be obtained by adopting different culture media through the propagation steps of sterile sowing, clumpy bud proliferation, strong seedling culture and sterile seedling transplanting. The method has the advantages of being high in seed germination rate, rapid in seedling formation, good in seedling quality, low in cost and the like, the transplanting survival rate of seedlings is high, and an effective way can be provided for production of Mongolian orchid seedlings.

Description

technical field [0001] The invention relates to the field of rapid propagation of orchid plants, in particular to a rapid propagation method and culture medium of Mengzilan. Background technique [0002] Mengzilan Mengzia foliosa (King&Pantl.) W.C.Huang, Z.J.Liu&C.Hu, orchidaceae perennial herb, distributed in southern Yunnan, China, hillside forests of Thailand. (Remarks: The original name is Huabaiji. The research results on the phylogeny of the Orchidaceae Longzuilan family where Huabaiji is located show that Huabaiji does not belong to the genus Baiji, and a new genus-Mengzilan should be established separately. International Plant The research results were published online in the academic journal Molecular Phylogenetics and Evolution, Huabai and changed its name to Mengzilan). [0003] The wild resources of Mengzilan (formerly known as Huabaiji) are rare and have been included in the National Rare and Endangered Plants (Endangered, EN) (List of Threatened Species of Hig...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 邵丽黄卫昌倪子轶曾歆花胡超蒋凯
Owner SHANGHAI CHENSHAN BOTANICAL GARDEN
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