Rapid propagation method and culture medium for Mongolian orchid

A technology for multiplying culture medium and culture medium, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as scarcity, and achieve the effects of good seedling quality, high seed germination rate, and fast seedling formation.

Pending Publication Date: 2022-05-13
SHANGHAI CHENSHAN BOTANICAL GARDEN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the scarcity of wild material resources in Mengzil

Method used

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  • Rapid propagation method and culture medium for Mongolian orchid
  • Rapid propagation method and culture medium for Mongolian orchid
  • Rapid propagation method and culture medium for Mongolian orchid

Examples

Experimental program
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Example Embodiment

[0032] implementation plan:

[0033] S1 Preparing materials: Select healthy orchid plants, artificially pollinate them when they bloom, and use the seeds in the fruits that have developed and uncracked after pollination for sowing. The number of days after pollination is the maturity of the seeds.

[0034] S2 Aseptic sowing: Rinse Mengzi orchid capsules under water flow for 20 minutes, soak them in 75% alcohol for 30s on the ultra-clean workbench, shake and disinfect with 0.1% mercury chloride solution containing a little Tween 20 for 12 minutes, and rinse with sterile water for 4 Second, filter paper to dry the surface water of the capsule. The capsules are then cut open and the seeds are inoculated onto the prepared germination medium.

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Abstract

The invention relates to the field of orchid rapid propagation, and discloses a Mongolian orchid rapid propagation method and a culture medium, and the method comprises the following steps: S1, material preparation, S2, aseptic sowing, S3, clumpy bud proliferation, S4, rooting and seedling strengthening, and S5, transplanting. According to the method, the Mongolian orchid seeds are used as explants, and a large number of seedlings can be obtained by adopting different culture media through the propagation steps of sterile sowing, clumpy bud proliferation, strong seedling culture and sterile seedling transplanting. The method has the advantages of being high in seed germination rate, rapid in seedling formation, good in seedling quality, low in cost and the like, the transplanting survival rate of seedlings is high, and an effective way can be provided for production of Mongolian orchid seedlings.

Description

technical field [0001] The invention relates to the field of rapid propagation of orchid plants, in particular to a rapid propagation method and culture medium of Mengzilan. Background technique [0002] Mengzilan Mengzia foliosa (King&Pantl.) W.C.Huang, Z.J.Liu&C.Hu, orchidaceae perennial herb, distributed in southern Yunnan, China, hillside forests of Thailand. (Remarks: The original name is Huabaiji. The research results on the phylogeny of the Orchidaceae Longzuilan family where Huabaiji is located show that Huabaiji does not belong to the genus Baiji, and a new genus-Mengzilan should be established separately. International Plant The research results were published online in the academic journal Molecular Phylogenetics and Evolution, Huabai and changed its name to Mengzilan). [0003] The wild resources of Mengzilan (formerly known as Huabaiji) are rare and have been included in the National Rare and Endangered Plants (Endangered, EN) (List of Threatened Species of Hig...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 邵丽黄卫昌倪子轶曾歆花胡超蒋凯
Owner SHANGHAI CHENSHAN BOTANICAL GARDEN
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