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Method for dissolving arsenic sulfide minerals and use thereof as therapeutic agents

An arsenic sulfide, mineral technology, applied in a new application field in research, can solve the problems of inability to induce promyelocytic leukemia-PML protein degradation, insignificant effect of virus escape latency, poor water solubility, etc.

Pending Publication Date: 2022-05-17
安德里亚·萨瓦里诺 +5
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although realgar contains arsenic, its effect on viral escape latency is very insignificant because: 1) Realgar has poor water solubility, which hinders in vitro testing of some parameters of the solute that would be useful for a substance as a potential HIV therapeutic candidate while generally accepted parameters; 2) realgar solutes are not well characterized: especially in acidic environments, arsenic can be oxidized to As(V), which is less biologically active than its trivalent counterpart [O'Day et al. 2006] (for example, As(V) failed to induce the degradation of the promyelocytic leukemia-PML protein, a key target of arsenic's anticancer and anti-HIV latency effects (see Shytaj et al., EMBO J. 2020))

Method used

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  • Method for dissolving arsenic sulfide minerals and use thereof as therapeutic agents
  • Method for dissolving arsenic sulfide minerals and use thereof as therapeutic agents
  • Method for dissolving arsenic sulfide minerals and use thereof as therapeutic agents

Examples

Experimental program
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Effect test

Embodiment

[0054] Orpiment induces dose-dependent reactivation of HIV latency

[0055] Orpiment (Kremer Pigmente, Aichstetten, Germany) was dissolved with NaOH solution (pH: 14) and sterile filtered. Latency-infecting T cell lines J Lat 9.2, 6.3 and 8.4 [Jordan et al., 2003] were used to test the effect of orpiment on HIV reactivation from latency. Similar to the J-lat 9.2 clone, the 6.3 and 15.4 clones (obtained through the NIHAIDSReagent Program) have one copy of latent HIV DNA per cell, and HIV transcription can be detected by GFP expression. In the absence of any stimulation, only a small fraction (usually Figure 4 ). However, after 48 h of orpiment treatment, GFP expression was increased at all concentrations used, with progressively higher levels of reactivation with increasing concentrations of orpiment ( Figure 4 ).

[0056] Notably, the HIV reactivation effect of orpiment was inhibited when cells were co-incubated with 10 mM glutathione precursor N-acetylcysteine ​​(NAC), s...

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Abstract

A method of completely dissolving mineral and / or powdered realgar (tetraarsenic tetrasulfide) in an aqueous solution. The method comprises the following steps: incubating realgar in an acidic environment for 24 hours, diluting the material from the incubating step in NaOH with the pH value of 13-14, and further diluting the material from the dilution in NaOH with the pH value of 12-13.

Description

[0001] This application claims priority to U.S. Provisional Application No. 62 / 878,765, filed July 26, 2019, and U.S. Provisional Application No. 62 / 904,853, filed September 24, 2019, both of which are incorporated by reference Incorporated into this article as a whole. [0002] The present invention at least relates to a method for treating various diseases by completely dissolving realgar (tetraarsenic tetrasulfide) in compliance with green chemical standards. For example, the present invention relates, at least for the first time, to the effect of orpiment on reactivating HIV from latency, thereby providing a new application of this arsenic mineral in research for the treatment of HIV / AIDS. Background technique [0003] Realgar is a kind of tetraarsenic tetrasulfide (As 4 S 4 )mineral. It occurs in the ore as red crystals and powder, which take on a characteristic orange color. Realgar was used as a paint during the Renaissance and Baroque periods [O'Day 2006]. Realgar...

Claims

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Application Information

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IPC IPC(8): A61K33/36A61P35/00
CPCA61K33/36A61K31/203A61P35/00A61P31/18A61K2300/00
Inventor 安德里亚·萨瓦里诺伊尔特·卢卡·希塔吉博亚娜·卢西奇玛丽娜·卢西奇马哈茂德·埃尔赫夫纳维利绍姆瓦·恩德洛武
Owner 安德里亚·萨瓦里诺
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