Integrated handheld digital nucleic acid detector and nucleic acid detection method

Abstract

The invention discloses an integrated handheld digital nucleic acid detector and a nucleic acid detection method. The nucleic acid detector comprises a chip sample adding and packaging system, a thermal circulation system and a detection and analysis system which are integrated into a whole, the chip sample adding and packaging system comprises a microporous dPCR chip and a packaging cover plate for packaging the microporous dPCR chip; the thermal circulation system is used for performing thermal circulation on the microporous dPCR chip; and the detection and analysis system is used for shooting a fluorescence image of the microporous dPCR chip after the thermal cycle is finished, and processing and analyzing the fluorescence image. A chip sample adding and packaging system, a thermal circulation system and a detection and analysis system are miniaturized and integrated into one instrument, so that the instrument structure is simplified, and the instrument cost is reduced; the invention further provides a flexible packaging process of the microporous dPCR chip based on the PDMS and Parylene C multilayer structure, and the problems of sample evaporation and cross contamination caused by mineral oil liquid sealing of the microporous digital PCR chip are solved.

Description

Technical field [0001] The present invention relates to the field of nucleic acid detection technology, in particular to an integrated handheld digital nucleic acid detector and nucleic acid detection method. Background [0002] Polymerase Chain Reaction (PCR), which specifically amplifies nucleic acids in vitro, is the gold standard for nucleic acid testing. For double-stranded nucleic acids, deoxyribonucleic Acid (DNA), the PCR process consists of two main parts: hot initiation and thermal cycling. Thermal initiation is the activation of polymerase activity in the master mix at high temperatures (about 95 °C); thermal cycling is the initiation of DNA replication under the action of the polymerase. A thermal cycle typically consists of three biological processes: high temperature denaturation, low temperature annealing, and medium temperature extension. During denaturation (about 95 °C), the hydrogen bonds between the internal strands of double-stranded DNA are broken, and one D...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide an integrated handheld digital nucleic acid detector and nucleic acid detection method to reduce the volume of the detection instrument and solve the problems of high detection cost and long detection time in the existing detection

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