KASP marker primer and kit for identifying imidazolinone herbicide-resistant rape and application of KASP marker primer and kit

A technology for labeling primers and rapeseed, applied in the field of crop molecular genetics and breeding, can solve the problems of low throughput, complex technology and high cost of SSCP or CAPS labeling, and achieve the effects of reducing soil residues, accurate detection results, and accelerating the selection process.

Pending Publication Date: 2022-05-27
HUAZHI RICE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the multi-copy characteristics of the AHAS gene, the molecular markers for screening related resistant rapeseed in articles and patents are based on the SSCP or CAPS markers at the above SNP sites, which avoids the interference of multiple copies to a certain extent, but the SSCP or CAPS markers Low throughput, high cost, complex technology, and time-consuming, so general breeding seldom uses SSCP or CAPS markers for screening, but directly screens with herbicides

Method used

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  • KASP marker primer and kit for identifying imidazolinone herbicide-resistant rape and application of KASP marker primer and kit
  • KASP marker primer and kit for identifying imidazolinone herbicide-resistant rape and application of KASP marker primer and kit
  • KASP marker primer and kit for identifying imidazolinone herbicide-resistant rape and application of KASP marker primer and kit

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Embodiment Construction

[0063] In order to make those skilled in the art understand the technical solutions of the present invention more clearly, the following examples are now given for illustration. It should be noted that the following examples do not limit the protection scope of the present invention.

[0064] The reagents, methods and equipment used in the following examples are all conventional reagents, methods and equipment in the technical field. The test methods that do not specify specific experimental conditions in the following examples are usually in accordance with conventional experimental conditions or in accordance with experimental conditions suggested by the manufacturer.

[0065] The reagents and consumables supporting the Douglas Array Tape genotyping platform used in the following examples were purchased from LGC (Laboratory of the Government Chemist) in the United Kingdom.

[0066] Design of KASP primers

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Abstract

The invention discloses a KASP marker primer for identifying imidazolinone herbicide-resistant rape, a kit and application of the KASP marker primer and the kit, and relates to the technical field of molecular genetic breeding of crops. The KASP marker primer group disclosed by the invention comprises a KASP marker primer group BN-PM1.1 and a KASP marker primer group BN-PM1.2 which are used for detecting an AHSH1 gene, and a KASP marker primer group BN-PM2.1 and a KASP marker primer group BN-PM2.2 which are used for detecting an AHSH3 gene. The KASP marker primer group is high in specificity, good in quality, high in sensitivity and high in resolution, and can accurately, quickly, efficiently and specifically identify the variation sites of PM1 and PM2 in the AHAS1 gene and the AHAS3 gene of the rape.

Description

technical field [0001] The present invention relates to the technical field of crop molecular genetics and breeding, in particular to KASP marker primers, kits and applications for identifying imidazolinone herbicide-resistant rape. Background technique [0002] Rapeseed is a major oil crop, and weeds are very harmful to rapeseed production. Chemical pesticides and herbicides are often used in production to remove weeds in rapeseed fields. Canola producing countries such as Canada and Australia have promoted and used herbicide-resistant rape on a large scale. Compared with rape without herbicide resistance, herbicide-resistant rape can improve the control efficiency of chemical pesticides on weeds in rape planting and reduce the control efficiency. Weed costs, reduce environmental damage, and make canola yields or economic returns greater. The selection and breeding of rapeseed varieties with resistance to specific herbicides is of great significance to the production of ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/6895C12Q1/6858
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2563/107Y02A50/30
Inventor 雷雨婷唐顺学田冰川谢心悦
Owner HUAZHI RICE BIO TECH CO LTD
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