Method for coating magnetic bead fishing traditional Chinese medicine active ingredients with cell membranes based on electroporation and application of method

Abstract

The invention discloses a method for coating magnetic bead fishing traditional Chinese medicine active components with cell membranes based on electroporation. The method comprises the following steps: 1, synthesizing Fe3O4 coated BSA-RDG magnetic nanoparticles by adopting coating and modification; 2, preparing a complete macrophage membrane coated nano magnetic bead by adopting electroporation; 3, fishing traditional Chinese medicine active ingredients; the invention also discloses application of the method in active ingredients of the fishing monkshood. According to the method, the Fe3O4 (at) BSA-RGD magnetic nanoparticles with good biocompatibility and membrane targeting are synthesized and are co-cultured with target cell macrophages, then electroporation is carried out, a complete macrophage membrane coated nano magnetic bead system is constructed, the structural integrity of a cell membrane is guaranteed, the outer surface of the cell membrane faces outwards, compared with a traditional coating method, the directions of the outer surface and the inner surface of the membrane are not distinguished, and the application range is wide. The quantity of target spots on the surface of the fishing carrier is increased, and the accuracy and sensitivity of screening active ingredients of traditional Chinese medicines are improved; according to the application, the anti-inflammatory active ingredients in the radix aconiti lateralis praeparata can be fished.

Description

Technical field [0001] The present invention belongs to the field of traditional Chinese medicine active ingredient screening technology, specifically relates to a method of fishing for the active ingredient of traditional Chinese medicine based on electroporation of cell membrane-coated magnetic beads and its application. Background [0002] Traditional Chinese medicine active ingredient studies are usually determined using bio-effect-oriented systematic isolation combined with pharmacological experiments. The components of traditional Chinese medicine are complex, and the content of active ingredients is often low, so its separation and analysis operation is cumbersome, the workload is large, the cycle is long, the success rate is low, and the essence of the overall role of "multi-component-multi-target" of traditional Chinese medicine is decomposed and ignored. [0003] Finding rapid screening methods and techniques for active ingredients has always been the focus of attention...

AI Technical Summary

Problems solved by technology

Although the traditional method can effectively coat the cell membrane on the surface of magnetic nanospheres, it still faces the following two problems: one is that the ultrasonic treatment / mechanical force destroys the integrity of the cell membrane and reduces the function of the cell membrane, which may cause The change or even denaturation of membrane protein conformation reduces the accuracy of drug screening results; second, the physical adsorption process cannot distinguish the inner / outer surface of the cell membrane, resulting in unclear drug screening sites, and at the same time, the receptors on the inner / outer surface of the cell membrane simultaneously Existence, reduced drug screening sites per unit area, reducing the sensitivity of related screening methods

Aconite can warm and invigorate kidney yang, dispel wind and disperse cold, and has pharmacological activities such as anti-inflammation and analgesia. It is clinically used to treat arthritis, but its anti-inflammatory active ingredients are still unclear

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