Method for screening double-antibody sandwich nano antibody pair based on lupin allergen Lup an1 nano antibody

A nanobody and double antibody sandwich technology, applied in the field of immunodetection, can solve the problems of long period of screening and antibody preparation, and achieve the effect of simple operation and short time-consuming.

Pending Publication Date: 2022-06-07
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The development of immunoassay technology for traditional food allergen components is based on the screening and preparation of monoclonal or polyclonal ant

Method used

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  • Method for screening double-antibody sandwich nano antibody pair based on lupin allergen Lup an1 nano antibody
  • Method for screening double-antibody sandwich nano antibody pair based on lupin allergen Lup an1 nano antibody

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Embodiment Construction

[0019]Unless defined, the technical terms used in the following embodiments have the same meaning as those generally understood by those skilled in the art to which the present invention belongs. The test reagents used in the following embodiments, unless otherwise specified, are conventional biochemical reagents; The experimental methods, unless otherwise specified, are conventional methods.

[0020] The present invention is described in detail below in conjunction with an embodiment.

[0021] A colloidal gold immunochromatography test strip method for lupine allergen Lup an1, including the following steps:

[0022] (1) Preparation of colloidal gold

[0023] The triangle bottles, rotors, etc. used in the preparation of colloidal gold are soaked in fresh chromic acid and double steamed water for 3 days and 1 day, respectively. Add enough double steamed water to the treated triangle bottle (including the rotor), heat and boil for 5 minutes and then discard. Add 100ml of double dist...

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Abstract

The invention provides a method for screening a double-antibody sandwich nano antibody pair based on a lupin allergen Lup an1 nano antibody. The method comprises the following steps: (1) preparing colloidal gold; (2) preparing a gold-labeled nano antibody; and (3) screening the double-antibody sandwich antibody pair by adopting an immunochromatography test strip method. According to the lupin allergen Lup an1 nano antibody, a colloidal gold immunochromatography test strip method is used for screening double-antibody sandwich nano antibody pairs, and a screening method for capturing antibodies and detecting antibodies in a double-antibody sandwich detection system, which is simple and convenient to operate and short in time consumption, can be developed and is used for constructing a subsequent method for detecting allergens in food.

Description

Technical field [0001] The present invention belongs to the field of immunoassays, in particular to a method based on a lupine allergen Lup an1 nano antibody using colloidal gold immunochromatography test strip method to screen double anti-sandwich nano antibody pairs. Background [0002] Immunoassays for traditional food allergen components develop screening and preparation based on monoclonal or polyclonal antibodies, which require injection of high-purity allergen proteins in animal immunization processes and a long cycle of screening and preparation of antibodies. A heavy chain only antibodies (HCAbs) are naturally present in the peripheral blood of camel-derived animals, and compared with traditional monoclonal antibodies, heavy chain antibodies naturally lack light chains and heavy chain first constant regions (CH1). Cloning and expressing the heavy chain variable region of the heavy chain antibody obtains the antigen recognition and binding domain of the heavy chain antibo...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/543G01N33/58
CPCG01N33/558G01N33/54346G01N33/587G01N33/581G01N2333/415
Inventor 张川王硕胡耀中陆旸
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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