The invention provides essence for turning white hair into black hair and application thereof, and relates to the field of cosmetics. The essence is specifically prepared from the following raw materials: EDTA (Ethylene Diamine Tetraacetic Acid) disodium, hydroxypropyl methyl cellulose, sodium pyrosulfite, a larch wood extract, acetyl tyrosine, a chlorella/lupin protein fermentation product, isopentyl glycol, a tea leaf extract, glycine, a cell phospholipid aqueous solution, whey protein, an apple fruit cell culture extract and a wild strawberry fruit extract. The composition is prepared from a wild soybean extract, a polygonum multiflorum extract, a ganoderma atrum extract, a black mulberry fruit extract and a sesame seed extract. The finally prepared essence has the effects of stimulating stem cell proliferation, stimulating generation of melanin in hair follicles, stimulating anti-oxidation defense of the hair follicles, reactivating generation of melanin in gray/white hair and the like. The composition can be applied to white hair blackening essence, anti-ash/white hair lotion, anti-white hair shampoo, natural black hair mask, beard blackening lotion, hair composite spray and premature white/white hair gel.