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Simple long-term preservation method for small insect impregnated specimens

A long-term preservation and insect technology, which is applied in the field of simple long-term preservation of small insect-dipped specimens, can solve problems such as specimen damage, poor sealing performance of centrifuge tubes, and poor sealing performance, and save time.

Pending Publication Date: 2022-07-01
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, 2 ml or 5 ml centrifuge tubes sold on the market are mainly used for preservation of insect soaked specimens. The preservation solution uses 75% alcohol solution or 95% alcohol solution, but the centrifuge tubes currently on the market have poor sealability. Ethanol is also a very volatile solvent. In practice, it was found that the preservation solution in the 2 ml centrifuge tube was significantly reduced or evaporated after one year. It was necessary to re-open the cap to add the preservation solution, which was time-consuming and laborious, and it was easy to cause damage to the specimen; , the number of centrifuge tubes stored in the container is large, and it is difficult to find samples; if a completely sealed centrifuge tube is developed, the cost is high
In addition, since the container needs to be opened when taking and placing the centrifuge tube containing the specimen, the moisture in the air will enter the absolute ethanol in the container, which may affect the purity of the absolute ethanol in the centrifuge tube
[0003] In summary, the problem of the existing preservation of insect-infused specimens is: the preservation of insect-infused specimens in the 2 ml and 5 ml centrifuge tubes currently on the market, due to the poor sealing of the centrifuge tubes, the preservation solution ( 75% alcohol solution or 95% alcohol solution) is easy to volatilize, cannot be stored for a long time or permanently, and it is difficult to find specimens

Method used

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  • Simple long-term preservation method for small insect impregnated specimens
  • Simple long-term preservation method for small insect impregnated specimens
  • Simple long-term preservation method for small insect impregnated specimens

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1, the simple long-term preservation method of ant immersion specimen is carried out according to the following steps, such as Figure 1-3 :

[0026] Step 1: Immediately put the collected fresh ant specimens into a 2ml centrifuge tube containing 75% alcohol solution or 95% alcohol solution and bring them back to the laboratory, and then take them out in batches for cleaning with alcohol;

[0027] Step 2: Sort the cleaned ant specimens under a dissecting microscope, put the same species of ant specimens from the same collection site into the same centrifuge tube 1, add 75% alcohol solution or 95% alcohol solution to 4 / 5 height of the centrifuge tube , the ant specimens from the same nest are put into the same centrifuge tube separately, the writing label is put into it and numbered and recorded, and the lid is tightened, see figure 1 ;

[0028] Step 3: Collect discarded plastic mineral water bottles, rinse them with clean water and use them for later use; pu...

Embodiment 2

[0031] Embodiment two, the simple long-term preservation method of ant immersion specimen, adopts such as Figure 4-7 the storage device shown, proceed as follows:

[0032] Step 1: The collected fresh ant specimens are immediately put into a 2ml centrifuge tube containing 75% alcohol solution or 95% alcohol solution and brought back to the laboratory, and then taken out in batches for cleaning with alcohol solution;

[0033] Step 2: Sort the cleaned ant specimens under a dissecting microscope, put the same species of ant specimens from the same collection site into the same centrifuge tube 1, add absolute ethanol to 4 / 5 of the height of the centrifuge tube, and ant specimens from the same nest Put it into the same centrifuge tube separately, and write the label and put it into the number record;

[0034] Step 3: Insert the centrifuge tube 1 into the round hole on the tray 4. There are several round holes for inserting the centrifuge tube on the tray. The inner diameter of the...

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Abstract

The invention discloses a simple long-term preservation method for small insect immersed specimens, which comprises the following steps: putting collected fresh insect specimens into a centrifugal tube filled with an alcoholic solution, adding the alcoholic solution to 4 / 5 height of the centrifugal tube, and putting a writing label into the centrifugal tube; classifying under an anatomical lens, and putting the same species of insects in the same collection place into the same centrifugal tube; after classification is completed, the centrifugal tubes in the same collection place are put into the same large container capable of being sealed, then the sealed container is stored in a dark place, and the alcoholic solution in the container is added regularly. According to the invention, the problem of specimen decay caused by volatilization of the preserving fluid due to poor sealing performance of the centrifugal tube is solved, so that the insect impregnated specimen can be well preserved for a long time or permanently, and can be quickly found according to the label identification.

Description

technical field [0001] The invention relates to the technical field of preservation of insect specimens, in particular to a simple and long-term preservation method for immersed specimens of small insects. Background technique [0002] At present, the preservation of insect immersion specimens mainly uses 2 ml or 5 ml centrifuge tubes sold on the market, and the preservation solution uses 75% alcohol solution or 95% alcohol solution. Ethanol is also a very volatile solvent. In practice, it was found that the preservation solution in the 2 ml centrifuge tube was significantly reduced or evaporated completely after 1 year. It was necessary to re-open the cap to add the preservation solution, which was time-consuming and labor-intensive, and easily damaged the specimen. , the number of centrifuge tubes stored in the container is large, and it is difficult to find specimens; if a completely sealed centrifuge tube is developed, the cost is high. In addition, since the container ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00
CPCA01N1/00
Inventor 刘霞徐正会
Owner SOUTHWEST FORESTRY UNIVERSITY