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Streptomyces allogeri BC1 and application thereof

A technology of Streptomyces allogica and Streptomyces, applied in the direction of application, bacteria, fungicides, etc., can solve problems such as pesticide residues, threats to human health, and damage to the ecological environment, and achieve low cost, good development and application prospects, and preparation methods simple effect

Active Publication Date: 2022-07-08
SUGARCANE RES INST OF YUNNAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the characteristics of low cost and quick effect of chemical control, chemical agents are mainly used in production to prevent and control sugarcane red rot and sugarcane brown streak disease. However, chemical control can easily lead to drug resistance of pathogenic bacteria, and can also cause pesticide residues and damage the ecological environment. and threats to human health

Method used

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  • Streptomyces allogeri BC1 and application thereof
  • Streptomyces allogeri BC1 and application thereof
  • Streptomyces allogeri BC1 and application thereof

Examples

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preparation example Construction

[0037] The present invention further provides a method for preparing a biological control fungicide, comprising the following steps:

[0038] (1) inoculate the described Streptomyces allogie BC1 in solid culture and carry out activation cultivation, obtain activated bacterial strain;

[0039] (2) the activated bacterial strain obtained in step (1) is inoculated in a liquid medium and cultivated to obtain liquid seeds;

[0040] (3) inoculating the liquid seeds obtained in step (2) into a fermentation medium for fermentation, and the fermentation broth is a biological control fungicide.

[0041] In the present invention, the temperature of the activation culture in step (1) is 28-32°C; preferably 29-31°C; more preferably 30°C.

[0042] In the present invention, the time of activation and culture in step (1) is 6-8 days; preferably, it is 7 days.

[0043] In the present invention, the temperature of the culture in step (2) is 28-32°C; preferably 29-31°C; more preferably 30°C. ...

Embodiment 1

[0055] A preparation method of a biological control fungicide, the steps are as follows:

[0056] (1) Inoculate Streptomyces aloje BC1 in solid culture at 28°C for activation and culture for 6 days to obtain an activated strain;

[0057] (2) inoculating the activated strain obtained in step (1) in a liquid medium at 28° C. for 24 hours, and the rotating speed of the shaker is 150 r / min to obtain liquid seeds;

[0058] (3) the liquid seeds obtained in step (2) were inoculated into the fermentation medium for fermentation at 28°C for 48h, and the rotating speed of the shaker was 250r / min; in the fermentation process, the volume ratio of dissolved oxygen was controlled at 10%, and the pH was controlled at 7.2, The fermentation broth is the biological control fungicide, and the viable count of Streptomyces aloe vera BC1 in the fermentation broth is 2×10 10 pcs / mL;

[0059] The fermentation medium is soluble starch 10g / L, peptone 5g / L, yeast extract powder 4g / L, malt extract powd...

Embodiment 2

[0061] A preparation method of a biological control fungicide, the steps are as follows:

[0062] (1) Inoculate Streptomyces allogae BC1 in solid culture at 32°C for 8d activation and culture to obtain an activated strain;

[0063] (2) inoculating the activated strain obtained in step (1) in a liquid medium at 32° C. for 48 hours, and the rotating speed of the shaker is 250 r / min to obtain liquid seeds;

[0064] (3) the liquid seeds obtained in step (2) were inoculated into the fermentation medium for fermentation at 32° C. for 72 h, and the rotating speed of the shaker was 350 r / min; in the fermentation process, the volume ratio of dissolved oxygen was controlled to 40%, and the pH was controlled at 7.4, The fermentation broth is the biological control fungicide, and the viable count of Streptomyces aloe vera BC1 in the fermentation broth is 6×10 10 pcs / mL;

[0065] The fermentation medium is soluble starch 10g / L, peptone 5g / L, yeast extract powder 4g / L, malt extract powder...

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Abstract

The invention relates to the technical field of biological control of plant diseases, in particular to streptomyces allogeri BC1 and application thereof. The invention provides a strain of Streptomyces allorgeri BC1, and the Latin name of the Streptomyces allorgeri BC1 is Streptomyces allorgeri BC1. The preservation number is CGMCC No: 24639. The streptomyces allogeri strain BC1 is successfully separated from sugarcane leaves, is used for preventing and treating sugarcane fungal diseases, has an obvious inhibition effect on sugarcane pathogenic bacteria such as sugarcane pokkah boeng pathogen, sugarcane red rot pathogen, sugarcane brown streak pathogen and sugarcane leaf blight pathogen, especially has a good effect on the sugarcane red rot pathogen, and has a good application prospect in prevention and treatment of sugarcane fungal diseases. Good development and application prospects are realized. The streptomyces allogeri strain BC1 fermentation medium provided by the invention is simple in preparation method and low in cost.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to a strain of Streptomyces aloje BC1 and its application. Background technique [0002] Sugarcane is the most important sugar crop in the world, an important raw material and strategic resource for light industry, chemical industry and energy, and is widely grown in tropical and subtropical regions. Sugarcane red rot (sugarcane red rot) is one of the most serious fungal diseases on sugarcane. It can damage sugarcane throughout the growth period. Cane stem red rot) and leaf midrib (ie midrib red rot). After the sugarcane stem is damaged, the pathogenic bacteria secrete sucrose invertase, which reduces the purity of the cane juice and the sucrose content, resulting in a 29.1% reduction in sugarcane yield and a 30.8% loss in sucrose content. Severe damage to the sugarcane area can lead to almost 100% yield loss. [0003] Sugarcane brown stripe (sugarcane...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/28A01P3/00A01G7/06A01G13/00C12R1/465
CPCC12N1/20A01N63/28A01G7/06A01G13/00Y02A40/10
Inventor 李婕黄应昆王晓燕张荣跃单红丽李银煳李文凤
Owner SUGARCANE RES INST OF YUNNAN ACADEMY OF AGRI SCI
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