Cultivation method of selenium-enriched cordyceps militaris
A cultivation method and technology of Cordyceps militaris, which is applied in the field of Cordyceps militaris cultivation, can solve problems such as low absorption utilization rate, difficult absorption of Cordyceps militaris, adverse effects of inorganic selenium medium utilization of Cordyceps militaris, etc., to achieve induced absorption and accumulation, improve nutrition and Medicinal value, the effect of increasing the selenium content of Cordyceps sinensis
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Embodiment 1
[0024] Embodiment 1: the cultivation method of a kind of selenium-enriched Cordyceps militaris of the present invention, comprises the following steps:
[0025] S1. Activation and cultivation of strains: inoculate the strains of Cordyceps militaris in the activation medium for strains, and cultivate at a constant temperature of 21°C, humidity of 60-85%, and shading for 7 days; the activation medium for strains includes the following qualities Percentage of components: wheat 10%, wheat bran 4%, soybean 8%, sucrose 1%, peptone 1.5%, potassium dihydrogen phosphate 0.8%, triamine citrate 1.2%, selenium-enriched yeast powder 3%, the balance for water.
[0026] S2, liquid bacterial culture: select the product with better activity in step S1, inoculate it in the liquid bacterial culture medium, and shake it at a constant speed under the shaking speed of 120-150 times / min to carry out shaker culture 5-7 The liquid culture medium includes the following components by mass percentage: 1...
Embodiment 2
[0031] Example 2: The difference from Example 1 is:
[0032] The strain activation medium includes the following components by mass percentage: wheat 12%, wheat bran 12%, soybean 12%, sucrose 1.5%, peptone 1%, potassium dihydrogen phosphate 1.2%, triamine citrate 0.5%, rich Selenium yeast powder 2%, the balance is water.
[0033] The liquid culture medium includes the following components by mass percentage: soybean powder 18%, sucrose 6%, peptone 2.2%, potassium dihydrogen phosphate 1.6%, vitamin B 12 0.8%, selenium-enriched yeast powder 2.6%, oak leaf extract 1.2%, and the balance is water.
[0034] The fruiting body selenium-enriched medium includes the following components by mass percentage: wheat 12%, wheat bran 8%, sucrose 3.5%, molasses 2%, peptone 3%, potassium dihydrogen phosphate 1.2%, triamine citrate 1%, Selenium-enriched yeast powder 3%, amino acid chelated calcium 1.5%, and the balance is water.
[0035] After testing, the content of selenium in the fruiting ...
Embodiment 3
[0036] Example 3: The difference from Example 1 is:
[0037] The strain activation medium includes the following components by mass percentage: wheat 15%, wheat bran 8%, soybean 22%, sucrose 2%, peptone 2%, potassium dihydrogen phosphate 1%, triamine citrate 1.5%, rich Selenium yeast powder 5.5%, the balance is water.
[0038] The liquid culture medium includes the following components by mass percentage: soybean powder 20%, sucrose 4%, peptone 1.5%, potassium dihydrogen phosphate 2.3%, vitamin B 12 0.6%, selenium-enriched yeast powder 3.3%, oak leaf extract 2.1%, and the balance is water.
[0039] The fruiting body selenium-enriched medium includes the following components by mass percentage: wheat 15%, wheat bran 12%, sucrose 4%, molasses 1.5%, peptone 4%, potassium dihydrogen phosphate 0.8%, triamine citrate 0.5%, Selenium-enriched yeast powder 5%, amino acid chelated calcium 2%, and the balance is water.
[0040] After testing, the content of selenium in the fruiting bodi...
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