Sample diluent for colloidal gold method new coronavirus antigen detection card

Abstract

The invention discloses a sample diluent for a colloidal gold method new coronavirus antigen detection card. The sample diluent comprises 0.1%-2% of a Tris buffer solution, 0.5%-3% of NP40, 0.1%-5% of TritonX-100, 0.05% of Proclin300, 0.001%-2% of phospholipase, 0.001%-2% of trypsin, 0.001%-2% of collagenase, 0.001%-2% of alkaline phosphatase, 0.02% of EDTA (Ethylene Diamine Tetraacetic Acid), 0.8%-2% of NaCl and 0.01%-5% of casein. According to the invention, phospholipase and alkaline phosphatase which destroy cell membrane components are added into a traditional diluent, and the phospholipase and the alkaline phosphatase have a synergistic effect, so that the capability of destroying the outermost membrane structure of the novel coronavirus is remarkably improved, the exposure rate of nucleocapsid protein of the novel coronavirus is increased, the detection rate of a detection card is greatly increased, and the sensitivity is improved.

Description

technical field [0001] The invention relates to the technical field of antigen detection, in particular to a sample diluent used for a colloidal gold method new coronavirus antigen detection card. Background technique [0002] The new coronavirus antigen detection is detected on the test strip through the binding reaction of antigen and antibody, which is convenient and quick, and the result is usually available in 15-20 minutes. At present, the main methods for the detection of new coronavirus antigens are: colloidal gold method, fluorescence immunochromatography and latex method. Among them, the colloidal gold method is used for antigen detection in the vast majority. The colloidal gold method is that chloroauric acid (HAuCl4) can be polymerized into gold particles of a certain size under the action of reducing agents such as white phosphorus, ascorbic acid, sodium citrate, tannic acid, etc., and become a stable colloidal state due to electrostatic action. , forming a ne...

AI Technical Summary

Problems solved by technology

The colloidal gold method of the new crown antigen detection card is to detect the nucleocapsid protein of the new crown antigen. Compared with the nucleic acid detection method

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