Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Injection-type bone morphogenetic protein using fibrin as carrier

A technology of morphogenic protein and fibrin, applied in the field of protein injections, can solve the problems of high cost, limited scope of application, complicated operation, etc., and achieve the effect of simple operation, wide application range, and prevention of adhesion

Inactive Publication Date: 2005-05-11
FOURTH MILITARY MEDICAL UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing clinical methods are compounding bone morphogenetic protein on solid materials and implanting it into the wound site through surgery. The operation is complicated, the cost is high, and the scope of application is limited, especially for closed fractures and other unsightly fractures that occur most frequently in clinical practice. Difficult to apply in cases requiring surgery

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: According to the technical scheme of the present invention, the injection-type bone morphogenetic protein with fibrin as the carrier is prepared according to the following steps:

[0016] 1) Obtain healthy adult anticoagulant blood from the hospital blood bank / blood bank or aseptically collect the patient's autologous venous blood before treatment;

[0017] 2) Centrifuge at 3000RPM for 20 minutes to separate plasma, extract fibrinogen by refrigerated centrifugation, add bovine bone morphogenetic protein extract at a ratio of 2mg: 1mg (fibrinogen: bone morphogenetic protein); freeze-dry the above mixture at -40°C Make flocs for later use;

[0018] Bovine bone morphogenetic protein extract is a mixture of various bone morphogenetic proteins artificially extracted from fresh calf cortical bone according to conventional methods. Mouse muscle bag experiment confirmed that it has osteoinductive activity;

[0019] 3) The fibrinogen / bone morphogenetic protein mi...

Embodiment 2

[0021] Embodiment 2: According to the technical scheme of the present invention, the injection-type bone morphogenetic protein with fibrin as the carrier is prepared according to the following steps:

[0022] 1) Obtain healthy adult anticoagulant blood from the hospital blood bank / blood bank or aseptically collect the patient's autologous venous blood before treatment;

[0023] 2) Centrifuge at 3000RPM for 20 minutes to separate plasma, extract fibrinogen by refrigerated centrifugation, add bovine bone morphogenetic protein extract at a ratio of 4mg: 1mg (fibrinogen: bone morphogenetic protein); freeze-dry the above mixture at -40°C Make flocs for later use;

[0024] Bovine bone morphogenetic protein extract is a mixture of various bone morphogenetic proteins artificially extracted from fresh calf cortical bone according to conventional methods. Mouse muscle bag experiment confirmed that it has osteoinductive activity;

[0025] 3) The fibrinogen / bone morphogenetic protein mi...

Embodiment 3

[0027] Embodiment 3: According to the technical scheme of the present invention, the injection-type bone morphogenetic protein with fibrin as the carrier is prepared according to the following steps:

[0028] 1) Obtain healthy adult anticoagulant blood from the hospital blood bank / blood bank or aseptically collect the patient's autologous venous blood before treatment;

[0029] 2) Centrifuge at 3000RPM for 20 minutes to separate plasma, extract fibrinogen by refrigerated centrifugation, add bovine bone morphogenetic protein extract at a ratio of 5mg: 1mg (fibrinogen: bone morphogenetic protein); freeze-dry the above mixture at -40°C Make flocs for later use;

[0030] Bovine bone morphogenetic protein extract is a mixture of various bone morphogenetic proteins artificially extracted from fresh calf cortical bone according to conventional methods. Mouse muscle bag experiment confirmed that it has osteoinductive activity;

[0031] 3) The fibrinogen / bone morphogenetic protein mi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an injection-type bone morphogenetic protein with fibrin as a carrier, which is suitable for the treatment of clinical fractures, nonunions and bone defects. It is based on the fibrin isolated from the blood of patients or healthy blood donors as a carrier, and the compound bovine bone morphogenetic protein is directly injected percutaneously into the injured part to exert the sustained release effect of the carrier and the bone morphogenetic protein to promote new bone growth and Fracture healing and accelerated wound repair. The invention has no immunogenicity and rejection reaction, and no adverse reaction after being implanted in the body. Fibrin is in a liquid state before use, and can be rapidly solidified in the body and can be shaped arbitrarily. At the same time, the hemostatic and adhesive effects of fibrin are beneficial to prevent the formation of local hematoma and provide a good local environment for bone regeneration and healing. The present invention has the advantages of definite therapeutic effect, wide application range, simple and convenient operation, safety and reliability confirmed by animal experiment and clinical experiment.

Description

[0001] 1. Field [0002] The invention relates to a protein injection in the medical field, in particular to a bone morphogenetic protein that can be used for injection in the treatment of clinical fractures, nonunions, bone defects and other diseases. 2. Background technology [0003] The main role of bone morphogenetic proteins is to promote the recruitment and differentiation of undifferentiated mesenchymal and osteocyte cells into osteoblasts. Because of its high-efficiency osteoinductive activity, it has excellent clinical application prospects in promoting fracture healing and treating bone defects and nonunions. Most of the existing clinical methods are compounding bone morphogenetic proteins on solid materials and implanting them into traumatic sites through surgery. The operation is complicated, the cost is high, and the scope of application is limited, especially for closed fractures and other unsightly fractures that occur most frequently in clinical practice. Case...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61K38/43A61P19/08
Inventor 雷伟孙明林胡蕴玉
Owner FOURTH MILITARY MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com