Soybean phytophthora testing reagent kit and its test method

A detection kit, Phytophthora soybean technology, applied in the biological field, can solve the problems of long cycle and low sensitivity, and achieve the effect of high accuracy and high sensitivity
CN1203189CInactive Publication Date: 2005-05-25NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2005-05-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The detection kit for phytophthora.sojae of the invention belongs to the field of crop disease prevention and treatment and plant quarantine. A pair of specific oligonucleotide primers were designed according to the ITS gene sequence of Phytophthora sojae. The kit includes: 1mL detection solution includes: 0.05mmol Tris.Cl, 0.125mmol KCl, 0.005mmol MgCl 2 , 0.01mmoldNTPs, upstream and downstream primers 0.25mmol, 0.1mgBSA, Taq DNA polymerase 100 units, add ultrapure water to prepare 1mL detection solution. The Phytophthora soybean detection kit based on this primer has strong specificity, sensitivity and stability, and provides a rapid, sensitive and specific technical method for the detection of Phytophthora soybean. The picture shows the specific PCR product of Phytophthora sojae.
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Description

(1) Technical field

[0001] The detection kit for Phytophthora soybean and the detection method thereof of the present invention belong to the field of biotechnology. It is specially used for the high-sensitivity and rapid detection of Phytophthora sojae carried by soybeans imported and exported by customs, and can also be used for early diagnosis of soybean blight in the field and monitoring of pathogens. (2) Background technology

[0002] Soybean Phytophthora root rot is caused by Phytophothora sojae, which is a widely distributed and extremely serious soil-borne disease. It is the A1 entry plant quarantine object announced by our country. The disease was first discovered in Indiana, USA in 1948. After the public report in 1955, it was discovered successively in 15 countries including Brazil, Argentina and Canada, the main soybean producing countries in the world. In the three years from 1989 to 1991 alone, soybean blight caused 2.79 million tons of yield loss in 12 state...

Claims

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