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Process for preparing dual-layer combined chitosan-gelatin-mucinase scaffold material

A technology of hyaluronic acid and scaffold material is applied in the field of preparation of chitosan-gelatin-hyaluronic acid double-layer composite scaffold material and scaffold material for biomedical engineering tissue transplantation, and achieves the effect of good mechanical properties

Inactive Publication Date: 2005-07-13
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Process for preparing dual-layer combined chitosan-gelatin-mucinase scaffold material
  • Process for preparing dual-layer combined chitosan-gelatin-mucinase scaffold material
  • Process for preparing dual-layer combined chitosan-gelatin-mucinase scaffold material

Examples

Experimental program
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Embodiment 1

[0016] Get refined chitosan (deacetylation degree 85%, molecular weight 300000) 2 grams and gelatin 2 grams and put into 90 milliliters of concentrations and be 1% acetic acid aqueous solution, stir 4 hours to reach dissolving completely under 40 ℃, drop 10 milliliters 1 % hyaluronic acid aqueous solution, stirring rapidly for 2 hours. 0.25% EDC was added dropwise to obtain a cross-linked chitosan-gelatin-hyaluronic acid aqueous solution (A). Take 20 ml of solution A and add it to a 90mm polystyrene petri dish, and pre-freeze at -40°C for 24 hours; control the temperature of the drying chamber of the freeze dryer at -40°C, and move the petri dish containing the pre-frozen liquid into the drying chamber. After the temperature is balanced, turn on the air pump, keep the air pressure in the drying chamber less than 5000 Pascals for 48 hours, then heat to 40°C under normal pressure and bake for 2 hours to obtain the crude product of the porous material, wash the product with 5% so...

Embodiment 2

[0018] Get refined chitosan (deacetylation degree 85%) 2 grams and gelatin 2 grams and put into 90 milliliters concentration and be 1% acetic acid aqueous solution, stir at 40 ℃ for 4 hours to reach dissolving completely, dropwise add 10 milliliters of 1% transparent The aqueous solution of uronic acid was stirred rapidly for 2 hours to obtain the aqueous solution of chitosan-gelatin-hyaluronic acid (A). Take 20 ml of solution A and add it to a 90mm polystyrene petri dish, and pre-freeze at -40°C for 24 hours; control the temperature of the drying chamber of the freeze dryer at -40°C, and move the petri dish containing the pre-frozen liquid into the drying chamber. After the temperature is balanced, turn on the air pump, keep the air pressure in the drying chamber less than 5000 Pascals for 48 hours, then heat to 40°C under normal pressure and bake for 2 hours to obtain the crude product of the porous material, wash the product with 5% sodium hydroxide ethanol solution (50%) T...

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Abstract

The invention discloses a preparation method of a chitosan-gelatin-hyaluronic acid double-layer composite support material. The method includes preparing a solution from chitosan-gelatin, adding a cross-linking agent and injecting the solution into a mould, the liquid layer reaches 1-10mm, and the crude product is obtained through pre-freezing and vacuum freeze-drying, and the crude product is subjected to secondary freeze-drying. The stent material is obtained, and its main feature is: dissolve in 0.5%-3% acetic acid with chitosan / gelatin as 1 / 0.3-3, prepare solution A with a content of 0.5%-4%, add 0.1 %-2% hyaluronic acid aqueous solution, the content of which is not more than 10% of the total amount of chitosan gelatin, to obtain solution B, adding carbodiimide cross-linking agent to solution B, or forming solution B through lyophilization The scaffold is soaked in the cross-linking agent solution, the pre-freezing temperature is between -20°C and -200°C, and the freeze-drying is carried out at -40°C and 5Kpa. The cross-section of the bracket material of the invention is a double-layer composite structure, which can meet the requirements of co-cultivation of different cells.

Description

technical field [0001] The invention relates to a preparation method of a chitosan-gelatin-hyaluronic acid double-layer composite support material. The invention belongs to the scaffold material technology for biomedical engineering tissue transplantation. Background technique [0002] Existing natural polymer scaffolds are mainly collagen-based scaffolds, including collagen-chitosan and collagen-chondroitin sulfate-6 scaffold structures. The Apligral artificial skin manufactured by Organogenesis Company of the United States was approved by the FDA in 1998. This product originated from the successful cultivation of fibroblasts and keratinocytes on collagen-based scaffold materials by MIT 20 years ago. John F. Burke et al constructed biomaterial scaffolds with collagen and chitosan. The scaffold has good biocompatibility and is easy for cell growth, but has certain antigenicity and large shrinkage. [0003] Gelatin is mixed with sodium alginate o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/24C08L5/08
Inventor 姚康德毛津淑尹玉姬
Owner TIANJIN UNIV