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Lung cancer early stage serum specific protein and its application

A specific protein and serum technology, which is applied in the diagnostic kit containing specific protein in early stage serum of lung cancer, the use of specific protein in early stage serum of lung cancer in the diagnosis of lung cancer, new tumor markers in the field of early serum specific protein in lung cancer, can solve the lack of lung cancer Early detection methods and other issues

Inactive Publication Date: 2005-11-02
SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of satisfactory and effective means for early detection of lung cancer

Method used

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  • Lung cancer early stage serum specific protein and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Determination of Serum Specific Proteins in the Early Stage of Lung Cancer

[0066] 1.1 Preparation of serum

[0067] Take 5ml of peripheral blood from patients with lung cancer confirmed by clinical pathology and healthy controls, and refrigerate at 4°C for 2 hours before the serum precipitates. Serum was collected after centrifugation at 3000g for 15 minutes, and then stored at -80°C after aliquoting. It is used after diluting 40 times with NaAc binding solution for detection.

[0068] 1.2 Preparation of chip

[0069] Take a WCX2 chip from Ciphergen Biosystems, Inc., USA, and install it on an 8-well sampler for use.

[0070] Chip pre-balancing:

[0071] Add 200 μl of NaAc binding solution to each well, and incubate on a shaker at room temperature for 5 minutes. Discard binding solution and repeat 1 time.

[0072] Loading:

[0073] Take the diluted serum, add 200 μl to each well, and incubate on a shaker at room temperature for 1 hour. Serum was discarded.

[...

Embodiment 2

[0084] Positive Predictive Value, Negative Predictive Value, Sensitivity and Specificity of Detection of Lung Cancer Using Early Serum Specific Protein of Lung Cancer

[0085] screening test

Lung cancer cases (cases)

Normal population (example)

Total

Positive (+)

44

7

51

feminine(-)

8

35

43

total

52

42

94

[0086] According to the above table, the calculated sensitivity is the true positive rate: 84.62%

[0087] Specificity or true negative rate: 83.33%

[0088] Positive predictive value: 86.27%

[0089] Negative predictive value: 81.40%

Embodiment 3

[0091] The relationship between serum specific protein in early stage of lung cancer and other lung tumors and pulmonary inflammatory diseases:

[0092] According to the same method as in Example 1, the proteome spectra of the serum of patients with malignant mesothelioma (MM), tuberculosis, lobar pneumonia and other lung diseases were detected and compared.

[0093] As a result, the above three specific proteins have not been found to co-exist in the serum of patients with the above diseases.

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Abstract

A serum specific protein in early stage of lung cance, its application in diagnosing lung cancer, and its reagent kit for said diagnosis are disclosed.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular, the invention relates to a new tumor marker lung cancer early serum specific protein. The invention also discloses the use of the early stage serum specific protein of lung cancer in diagnosing lung cancer. The invention also provides a diagnostic kit containing the early serum specific protein of lung cancer. Background technique [0002] Primary pulmonary bronchial carcinoma, referred to as lung cancer (Lung cancer), is a common malignant tumor that originates in the bronchial epithelium at all levels. Lung tissue cells become cancerous under the stimulation of some factors, and abnormal hyperplasia and enlargement of lung tissue are its pathological characteristics. Cough, chest pain, hemoptysis, chest tightness, shortness of breath, wheezing, weight loss and cachexia are the main clinical manifestations. [0003] Lung cancer has become the malignant tumor with t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C07K14/765G01N33/38G01N33/574
Inventor 张胜年卢伟王文静项翠琴陆晔
Owner SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION