Plasmid of recombinant immunotoxin Rantes-DT390 aimed at activating Th1 cell, and its preparing method and use

Abstract

A new type of recombinant immunotoxin plasmid for activating Th1 cells, with the chemokine Rantes as the guiding molecule and the active fragment DT390 of diphtheria toxin as the toxin molecule, constructed by gene recombination. The preparation method of the immunotoxin plasmid comprises the steps of constructing the recombinant plasmid, preparing engineering bacteria transfected by the recombinant plasmid, and the like. The immunotoxin plasmid has strict targeting, and can specifically attack activated Th1 cells with DT390 through the guidance of Rantes, and induce specific immune tolerance, thereby avoiding non-selective killing of all T cells. It is especially suitable for the prevention and treatment of autoimmune diseases and organ transplantation rejection, and can also be applied to the treatment of certain tumors.

Description

technical field

[0001] The invention relates to a novel recombinant immunotoxin plasmid for specifically attacking and killing activated Th1 cells and a preparation method thereof. Background technique

[0002] Immunotoxins (Immunotoxins, ITs) are hybrid molecules formed by connecting biological toxins with targeting carriers (antibodies or cytokines), also known as biological missiles or targeting toxins. The earliest immunotoxins (i.e., the first generation immunotoxins) were chemical conjugates of carriers and toxin molecules. This type of immunotoxins has strong immunogenicity, large molecular weight, poor permeability, and it is difficult to form an effective concentration and stability at the target site. And the uniformity is poor, the toxic and side effects are strong, and it is difficult to produce on a large scale, thereby limiting its application. With the continuous development of modern life sciences, people have improved immunot...

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