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Transparent acid gel and its preparation method

The technology of hyaluronic acid and gel is applied in the field of hyaluronic acid gel and its preparation, which can solve the problems of white appearance, short storage period and high protein content of gel products, so as to improve product quality, prevent chain scission, The effect of product quality improvement

Inactive Publication Date: 2006-06-14
SHANDONG KAILEPU PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are two sources of existing hyaluronic acid raw materials, one is animal tissues, such as cockscombs, ox eyes, etc., which are usually obtained by extraction; the other source is prepared by bacterial fermentation, but no matter which method is used, the obtained transparent Alcohol raw materials contain a lot of impurities such as protein, if not treated, the appearance of the gel product will be white, opaque, and harmful bacteria are easy to grow, resulting in a short shelf life
[0004] Although U.S. Patent No. 5,827,937 discloses a method for preparing hyaluronic acid gel with 1,4-butanediol glycidyl ether as a cross-linking agent, it is activated at 40° C. for 4 hours, then diluted to 0.5-1%, and then The gel can only be obtained by distillation under reduced pressure. The international patent WO8600079 is to add 1,4-butanediol glycidyl ether to hyaluronic acid for cross-linking at 50°C to obtain hyaluronic acid gel. They all have high reaction temperature As a result, the hyaluronic acid raw material is broken and degraded, which has adverse effects on the product. At the same time, due to the improper selection of the ratio of the cross-linking agent to the raw material, the water absorption rate of the gel product is too high or too low, resulting in poor cushioning effect after the product is injected into the human body. question

Method used

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  • Transparent acid gel and its preparation method
  • Transparent acid gel and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Dissolve 10g of hyaluronic acid in 1000ml of water to form a 1% solution, add 5mg of BD-2 deproteinized product from Boda Company of Tianjin University, stir well, let stand until precipitation appears, and then filter. Add 3000ml of 95% edible ethanol to the filtrate, stir vigorously until a white precipitate appears, filter, and vacuum-dry the filter cake. 9.8 g of hyaluronic acid with a protein content of less than 0.1% were obtained.

[0030] Cross-linking reaction:

[0031] Example 1

[0032] Dissolve 2 g of deproteinized hyaluronic acid prepared in Example 1 in deproteinized pretreatment in 24 ml of 1% NaOH, add 40 mg of ethylene glycol glycidyl ether, react at 15 ° C for 2 hours, let stand overnight, and then use Soak in deionized water, adjust to neutral with HCl, after fully soaking, the weight of the water-absorbing gel is 1000g, so the water absorption rate formula Q = m 2 ...

Embodiment 2

[0034] Dissolve 2 g of the deproteinized hyaluronic acid prepared in Example 1 of the deproteinized pretreatment in 20 ml of 1.5% NaOH solution, add 100 mg of 1,4-butanediol glycidyl ether, stir well and place it at 20°C for 24 hours to react The product is placed in ion-free water, and HCl is added to adjust the pH value to neutrality. After fully soaking, the water-absorbing gel is 840g. From the water absorption formula, it can be seen that the resulting gel product is a hyaluronic acid gel with a water absorption rate of 420 times (see Table 1).

Embodiment 3

[0036] Dissolve 2 g of the deproteinized hyaluronic acid prepared in Example 1 of deproteinized pretreatment in 20 ml of 1% HCl solution, add 200 mg of 1,4-butanediol glycidyl ether, and react at 35° C. Place overnight, then put the reactant into deionized water, adjust neutrality with NaOH, after fully soaking, the water-absorbing gel weight is 700g, as can be seen from the water absorption formula, the resulting gel product is a hyaluronic acid gel with a water absorption rate of 350 times (see Table 1).

[0037] hyaluronic acid

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Abstract

The present invention relates to a kind of hyaluronic acid gel and its preparation method, it is to deproteinize the hyaluronic acid, then select the glycidyl ether accounting for 1-10% of the weight of the hyaluronic acid in acidic or alkaline environment at 15 Under the condition of -35°C, react at constant temperature for 2-24 hours to prepare hyaluronic acid gel. Because the present invention adds pre-treatment to the hyaluronic acid raw material, the prepared gel is colorless and transparent, and prevents the growth of some harmful bacteria, so it can be stored for a long time: and the range of water absorption rate of the product prepared by the present invention is the best: Simultaneously, the reaction condition of the present invention is mild, effectively prevents chain scission and degradation of the hyaluronic acid raw material, and improves product quality.

Description

technical field [0001] The invention relates to a hyaluronic acid gel and a preparation method thereof, more specifically to a hyaluronic acid gel and a method for preparing the hyaluronic acid gel by adding a cross-linking agent into hyaluronic acid. Background technique [0002] Hyaluronic acid is a straight-chain polymer polysaccharide in which β-D-N-acetylglucosamine and β-D-glucuronic acid are combined with each other. With species and organ specificity, the hyaluronic acid gel made of hyaluronic acid shows good compatibility with the body when transplanted or injected into the body as a filler, and has the functions of anti-wrinkle, breast enhancement, cushion filling, etc., and It has no side effects on the human body and is widely used in medical and beauty industries. [0003] There are two sources of existing hyaluronic acid raw materials, one is animal tissues, such as cockscombs, ox eyes, etc., which are usually obtained by extraction; the other source is prepar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L5/08C08J3/075
Inventor 王捷孟保东汪文继
Owner SHANDONG KAILEPU PHARMA
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