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Method for freeze-drying and ultra low temperature storing ginseng callus cell

A callus and cell technology, applied in the field of cell preservation of plant germplasm resources, can solve problems such as preservation methods, and achieve fast recovery growth, simple operation, and economical and reasonable effects

Inactive Publication Date: 2006-06-28
长春生物制品研究所有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has not been a technical means that is not only commonly used in laboratories, but also capable of large-scale and long-term stable preservation of plant germplasm, and some technical links still need to be further improved.
[0003] For many plant cells, such as ginseng callus cells, no preservation method has been proposed

Method used

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  • Method for freeze-drying and ultra low temperature storing ginseng callus cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of materials to be cryopreserved: in liquid suspension culture, the callus cells should be cultured for 5-7 days; in solid culture, the callus cells should be cultured for 10-15 days.

Embodiment 2

[0029] Preparation of protective agents: Take trehalose, sucrose, glucose, fresh skim milk, and proline to prepare No. 1-6 protective agents respectively, No. 1, 2, 4, 5, and 6 protective agents are dissolved in 67V liquid medium, No. 3 protective agents The agent is directly dissolved in fresh skimmed milk. Freeze-drying and cryopreservation were carried out respectively. The concentrations are as follows:

[0030] Protective agent No. 1: 5% DMSO, 10% glycerin

[0031] Protectant No. 2: 20% sucrose, 5% DMSO, 10% glycerin

[0032] No. 3 protective agent: fresh skimmed milk, 5% DMSO, 10% glycerin

[0033] Protective agent No. 4: 1.0M proline, 5% DMSO, 10% glycerin

[0034] Protective agent No. 5: 10% trehalose, 5% DMSO, 10% glycerin

[0035] No. 6 protective agent: 10% trehalose, 10% sucrose, 5% DMSO, 10% glycerin

Embodiment 3

[0037] Pre-cultivation: Take the materials to be frozen and pre-culture them in the medium added with trehalose, sucrose, glucose, fresh skim milk, and proline. Pre-cultivate in 67V medium of % DMSO and 5% trehalose for 3 days at 25°C, then pre-culture in 67V medium of 5% DMSO and 10% trehalose for 1 day at 4°C.

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Abstract

The present invention provides the method of freeze drying and ultralow temperature storing ginseng callus cell. Mycose is used as the protecting agent component for freeze drying and ultralow temperature storing ginseng callus cell. The present invention also relates to one kind of preserved ginseng callus cell. The method of the present invention has obvious effect in preserving ginseng callus cell.

Description

Technical field: [0001] The invention relates to cell preservation of plant germplasm resources, in particular to preservation of ginseng callus cells. The invention relates to a freeze-drying method and a cryopreservation method of ginseng callus cells, and freeze-dried ginseng callus cells. Background technique: [0002] Plant genetic diversity is a key factor for protecting the human living environment and maintaining the sustainable development of agriculture. How to preserve and utilize species diversity is one of the major issues facing society today. Plant tissue culture technology and cryopreservation technology play an important role in the long-term preservation of plant germplasm, and some of these methods have begun to be applied. So far, there has not been a technical means that is not only commonly used in laboratories, but also capable of large-scale and long-term stable preservation of plant germplasm, and some technical links still need to be further improv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00A01N3/00
CPCA01N3/00A01H4/001A01H4/005A01H4/002
Inventor 盛军刘晓宇李娟刘丹回悦君郭桥于海鹏王志武张雪梅蔡伟明
Owner 长春生物制品研究所有限责任公司
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