Nano immune magnetic particle, preparing process and application
A technology of nano-magnetic particles and magnetic particles, which is applied in the field of preparation of nano-immune magnetic particles, can solve problems such as the absence of nano-immune magnetic particles, and achieve good dispersion and suspension stability, specific separation, and weak interaction effects
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Embodiment 1
[0040] (1). Preparation of dextran magnetic nanoparticles:
[0041] ①Add 0.7mol / L FeCl 3 ·6H 2 O solution 16ml and 1.6mol / L FeCl 2 4H 2 Mix 4ml of O solution, add dropwise to 20ml of 50% (w / v) dextran T-40 solution under nitrogen atmosphere, put the mixed solution in a water bath at 60°C for 15 minutes, and pour it into the mixed solution under vigorous stirring Add 40ml of 5mol / L ammonia water dropwise, and finish dropping in 15 minutes. The temperature is still kept at 60°C, and nitrogen gas is always passed to prevent oxidation to make a suspension;
[0042] ②The resulting suspension was adjusted to pH 7.0 with acetic acid;
[0043] ③The aggregates in the suspension were removed by centrifugation at 600×g for 15 minutes; the free dextran was separated from the dextran nano-magnetic particles by polyacrylamide dextran (Sephacryl) S-300 gel chromatography column filtration, The elution equilibrium solution is 0.01mol / L phosphate buffer (pH 7.4), and the collected particl...
Embodiment 2
[0049] (1). Preparation of dextran magnetic nanoparticles:
[0050] ①Add 0.5mol / L FeCl 3 ·6H 2 O solution 12ml and 1.4mol / L FeCl 2 4H 2 Mix 3ml of O solution, add dropwise to 15ml of 30% (w / v) dextran T-40 solution under nitrogen atmosphere, put the mixed solution in a water bath at 70°C for 10 minutes, and pour it into the mixed solution under vigorous stirring Add 30ml of 3mol / L ammonia water dropwise, and finish dropping in 10 minutes. The temperature is still kept at 70°C, and nitrogen gas is always passed to prevent oxidation to make a suspension;
[0051] ② The resulting suspension was adjusted to pH 6.0 with dilute hydrochloric acid;
[0052] ③The aggregates in the suspension were removed by centrifugation at 600×g for 10 minutes; the free dextran was separated from the magnetic dextran particles by polyacrylamide dextran (Sephacryl) S-300 gel chromatography column filtration, The elution equilibrium solution is 0.005mol / L phosphate buffer (pH6.0), and the collecte...
Embodiment 3
[0057] (1). Preparation of dextran magnetic nanoparticles:
[0058] ①Add 1.2mol / L FeCl 3 ·6H 2 O solution 10ml and 2.5mol / L FeCl 2 4H 2 Mix 3ml of O solution, add dropwise to 13ml of 70% (w / v) dextran T-40 solution under nitrogen atmosphere, place the mixed solution in a water bath at 50°C for 30 minutes, and pour it into the mixed solution under vigorous stirring Add 26ml of 7mol / L ammonia water dropwise, and finish dripping in 30 minutes. The temperature is still kept at 50°C, and nitrogen gas is always passed to prevent oxidation to make a suspension;
[0059] ②The resulting suspension was adjusted to pH 8.0 with dilute hydrochloric acid;
[0060] ③The aggregates in the suspension were removed by centrifugation at 600×g for 20 minutes; the free dextran was separated from the magnetic dextran particles by polyacrylamide dextran (Sephacryl) S-300 gel chromatography column filtration, The elution equilibrium solution is 0.02mol / L phosphate buffer (pH8.0), and the collecte...
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