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Extraction and purification of blue berry and its product

A purification method and blue berry technology, applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of high cost, complicated process, and low purity of the extract, and achieve low cost, improved dissolution rate, high The effect of biological activity

Inactive Publication Date: 2006-10-11
贵州金桫椤科技农业发展有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The whole process of the extraction method is complex and costly, and the obtained extract has a lower purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Use 20 grams of blueberry fruit pomace as raw material, dry in a ventilated drying oven to remove moisture, put it into a flat-bottomed flask, add 2 times the volume, pH of 2, and a concentration of 30% ethanol solution, place it on a water bath, and place it in a water bath. Reflux extraction at 30°C for 0.5 hours, filter with a Buchner funnel and collect the filtrate and residue respectively. Put the residue into a flat-bottomed flask, add 2 times the volume of ethanol solution with a pH of 2 and a concentration of 30%, reflux extraction under the same conditions for 0.5 hours and then filter, combine the two filtrates and put them into a flat-bottomed flask, and then put Put it into a refrigerator at a temperature of 0°C and let it stand for 10 hours. Then, the refrigerator was taken out, filtered with a Buchner funnel, the filtrate was placed on a rotary evaporator, and concentrated at 50° C. for 10 hours to obtain 50 ml of a concentrated solution. After passing th...

Embodiment 2

[0021] Take 20 grams of blueberry fruit pomace as raw material, dry it in a ventilated drying oven to remove moisture, put it into a flat-bottomed flask, add 5 times the volume, PH is 3, and a concentration of 80% ethanol solution, put it on a water bath, and put it in a water bath. Reflux extraction at 50°C for 1 hour, filter with a Buchner funnel and collect the filtrate and residue respectively. Put the residue into a flat-bottomed flask, add 5 times the volume of ethanol solution with a pH of 3 and a concentration of 80%, reflux extraction under the same conditions for 1 hour and then filter, and combine the two filtrates. The filtrate was placed on a rotary evaporator and concentrated at 50° C. for 12 hours to obtain 150 ml of a concentrated solution. Put the concentrated solution into a flat-bottomed flask, put it into a refrigerator with a temperature of 4° C., and let it stand for 12 hours. Then, take out the refrigerator, filter with a Buchner funnel, pass the filtra...

Embodiment 3

[0023] Take 20 grams of blueberry pomace as raw material, dry it in a ventilated drying oven to remove moisture, put it into a flat-bottomed flask, add 10 times the volume, PH is 6, and the concentration is 60% ethanol solution, put it on a water bath, and put it in a water bath. Reflux extraction at 90°C for 2 hours, filter with a Buchner funnel and collect the filtrate and residue respectively. Put the residue into a flat-bottomed flask, add 10 times the volume of ethanol solution with a pH of 6 and a concentration of 60%, reflux extraction under the same conditions for 2 hours and then filter, and combine the two filtrates. Put the combined filtrate into a flat-bottomed flask, put it into a refrigerator with a temperature of 2° C., and let it stand for 11 hours. Then, the refrigerator was taken out, filtered with a Buchner funnel, the filtrate was placed on a rotary evaporator, and concentrated at 50° C. for 10-12 hours to obtain 200 ml of a concentrated solution. After pa...

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Abstract

The invention relates to a method for extracting and purifying blue berries and a product thereof. After the blue berries are subjected to acidified ethanol reflux extraction, impurity removal, concentration, ultrafiltration and drying, a crude extract can be obtained; adding a column loading process between ultrafiltration and drying can High-purity blue berry anthocyanins are obtained, and the anthocyanins content is greater than 35%. The method of the invention has the advantages of simple operation, low cost and safe production, and can significantly improve the purity of total anthocyanins in the extract. The product obtained by the method of the invention has nutritional value and pharmacological action, and has good biological activity, and is a valuable raw material for the production of health food and medicine.

Description

technical field [0001] The invention relates to a method for extracting and separating effective components in plants, in particular to a method for extracting effective components of anthocyanin compounds from blueberries, and belongs to the technical field of biopharmaceuticals. Background technique [0002] Blueberry (Vaccinium myrtillus L.) is a kind of health fruit rich in various nutrients. Its antioxidant capacity ranks first among fruits and vegetables. At present, a variety of bioactive components in blue berries, especially anthocyanins, are the hotspots of foreign research. [0003] Anthocyanins in blue berries are one of the best natural pigments that have been utilized so far. As a plant pigment, it conforms to the development trend of green food. [0004] In recent years, many literatures have reported the nutritional value and pharmacological effects of blue berry anthocyanins, mainly for the following points: 1. Promote the resynthesi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/065C07H1/08
Inventor 王伯初王亚洲李知敏
Owner 贵州金桫椤科技农业发展有限公司