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Method of counting keukocytes and leukocyte counter

A white blood cell counting and white blood cell technology, applied in the field of white blood cell counting, can solve the problem of no known and achieve the effect of accurate white blood cell counting

Inactive Publication Date: 2001-02-28
KOWA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no known method for counting leukocytes in which a cytolytic reagent that exposes the nuclei of leukocytes and lyses platelets or erythrocytes is used in order to lyse platelets or erythrocytes in platelet preparations or erythrocyte preparations

Method used

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  • Method of counting keukocytes and leukocyte counter
  • Method of counting keukocytes and leukocyte counter
  • Method of counting keukocytes and leukocyte counter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0119] Embodiments of the present invention will be described below. Example 1: White blood cell count in platelet product ə> dissolves platelets in platelet product solution

[0120] 15 μL of Triton X-100 surfactant was added at various concentrations to the platelet preparation solution so that the final concentration was 0.01 to 10%. Each platelet product solution added with Triton X-100 was stirred for 20 seconds with a vortex mixer (Applied Science Industries) to accelerate the exposure of leukocyte nuclei, while using an automatic blood cell counter (Sysmex (trade name), Toa Medical Electronics Co. ., Ltd.) measured the number of platelets in the platelet product solution to evaluate the dissolution of platelets by Triton X-100. Simultaneously, the light transmittance of the platelet preparation solution was measured with a spectrophotometer (Beckman).

[0121] The measurement results of the number of platelets in the solution of platelet products and their light trans...

Embodiment 2

[0129] The container in which the leukocytes are collected on the filter is placed on a leukocyte counting device with a CCD image processor, and the leukocytes collected on the bottom portion are detected by the CCD image processor and the number of the leukocytes is measured. Embodiment 1 The image of the bottom portion of the container can form an image in the image-capturing surface of the CCD image processor. Therefore, image-capturing surfaces etc. do not need to be scanned. Example 2: White blood cell count in erythrocyte preparation ə> lysed erythrocytes in solution of erythrocyte preparation

[0130] Add 15 μL of surfactant Triton X-100 at different concentrations to the red blood cell preparation solution so that the final concentration is 0.01 to 10%. Each erythrocyte preparation solution added with TritonX-100 was stirred with a vortex mixer (Applied Science Industries) for 20 seconds to accelerate the exposure of leukocyte nuclei, and an automatic hemocytometer (...

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Abstract

A leukocyte counting method which comprises adding a cytolytic agent to a platelet preparation solution or an erythrocyte preparation solution to solubilize the platelets or erythrocytes in the respective solution in a leukocyte container which comprises an opening section, a side section and a bottom section and wherein the whole or part of the side section is provided with a portion having a horizontal cross section progressively increasing in the direction from the bottom section toward the opening section, centrifuging the container to accumulate the leukocytes in the bottom section, and counting the leukocytes thus accumulated.

Description

technical field [0001] The present invention relates to a method for counting white blood cells and a device for counting white blood cells. In particular, the invention relates to methods and devices suitable for counting leukocytes in platelet preparations and red blood cell preparations. Background technique [0002] Platelet products and red blood cell products are mainly used to relieve thrombocytopenia and anemia, surgery and other diseases. In consideration of side effects and other factors, in order to ensure quality, it is best not to have white blood cells in platelet products and red blood cell products. Therefore, in order to control the quality, the quantity of the small number of leukocytes remaining in the platelet product or red blood cell product should be measured. [0003] Typically, white blood cells are counted in platelet and red blood cell preparations by exposing and staining the nuclei of the white blood cells. That is, leukocytes are aggregated w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N15/00G01N15/14
CPCG01N2015/008G01N1/30G01N2015/1486G01N15/1475Y10T436/107497Y10T436/101666G01N2015/016G01N15/1433G01N33/49
Inventor 薮崎克己
Owner KOWA CO LTD
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