58 results about "Leukocyte Counts" patented technology

The invention discloses a compound probiotic agent for improving immunity of dogs and reducing incidence of diarrhea as well as a preparation method and application thereof, and belongs to the field of the compound probiotic agent. The compound probiotic agent is prepared by mixing and compounding a lactobacillus plantarum KT-Lp9 microbial agent, a lactobacillus casei zhang microbial agent, a lactobacillus plantarum P-8 microbial agent, a bifidobacterium animalis subsp. lactis V9 microbial agent and a dilution carrier, wherein the quantity of viable bacteria of the four kinds of microbial agents is more than or equal to 2*10<11>CFU / g. The compound probiotic agent with different contents of viable bacteria only can be applied to 96 dogs at different ages for 60 days, so that the immunity of the test dogs can be improved, and the incidence of diarrhea and the sickness degree are reduced. Main representations are that the feed intake and growth rate of the dogs are increased, leukocyte counts, lymphocytes counts and neutrophil counts in blood are controlled, and the contents of four immunologic factors including immune globulin G, interleukin 6, interferon alpha and tumor necrosis factor alpha in the blood can be increased; and meanwhile, the content of secretory immunoglobulin A (SigA) in excrement is increased.

A method for improving the accuracy of leukocyte classifying result of blood sample comprises: A, in the counting process of leukocyte, adding hemolytic agent in two times, testing and obtaining a first leukocyte counting number after adding the hemolytic agent at first time and a first leukocyte histogram, and a second leukocyte counting number after adding hemolytic agent at second time, while the hemolytic dosage added in the first time makes red blood cell fragments left affecting the counting number of leukocyte in the blood sample, and the hemolytic dosage added in the second time makesno red blood cell fragments left to avoid affecting the counting number of leukocyte; B, according to the ratio between the first leukocyte counting number and the second leukocyte counting number, determining a leukocyte classification histogram, and classifying leukocytes. The method can confirm the accuracy of leukocyte counting number and hemoglobin test result, and can improve the classification accuracy of leukocyte of animal blood samples of dog, cat and the like.

A leukocyte counting method which comprises adding a cytolytic agent to a platelet preparation solution or an erythrocyte preparation solution to solubilize the platelets or erythrocytes in the respective solution in a leukocyte container which comprises an opening section, a side section and a bottom section and wherein the whole or part of the side section is provided with a portion having a horizontal cross section progressively increasing in the direction from the bottom section toward the opening section, centrifuging the container to accumulate the leukocytes in the bottom section, and counting the leukocytes thus accumulated.

A fermentation Chinese Caterpillar Fungus hypha micro-powder, its production and use are disclosed. It is cheap and has less medicine use account. It can be used to prevent or treat chronic bronchitis, cough, leukocyte reduction and nephropathy.

The invention discloses a disposable cell counting slip with reticle. The disposable cell counting slip is composed of an upper cover and a lower cover which are made of transparent materials in an injection molding mode. The upper cover and the lower cover are combined into a whole and form a closed hollow transparent box. Sample adding holes are reserved in the head of the upper cover. A detection region formed in a molding mode through a mold is arranged on the inner bottom face of the lower cover and divided into a plurality of independent detection areas. A standard counting reticle pattern which is formed in an injection molding mode and is coincident with the pattern of a counting chamber specified by the NBS is arranged in the center of each detection area. The detection areas are communicated with the corresponding sample adding holes in a capillary mode. The disposable cell counting slip is easy to use and operate, and time and labor are saved; the injuring and infecting risk generated during operation is reduced. The disposable cell counting slip can be used for a cell counting instrument and serves as an on-instrument consumable. A glass blood cell counting plate can be completely replaced, and the disposable cell counting slip is used for carrying out microscope counting on red blood cells and white blood cells in the human body in a clinical examination and is also often used for counting microorganisms such as cell culturing microorganisms, bacteria, fungi and yeasts.

A blood cell analyzer, a reticulocyte counting apparatus and a count correcting method are disclosed. The count correcting method comprises following steps: (1) carrying out a reaction between a fluorescent reagent and a blood sample to obtain a sample liquid; (2) feeding a proper amount of the sample liquid to an optical detection device for carrying out light irradiation and detecting optical information generated by various cells particles in the sample liquid; (3) obtaining an original reticulocyte counting value according to the optical information generated by the various cell particles, wherein the original reticulocyte comprises reticulocyte and leukocyte; and (4) performing the original reticulocyte counting value minus the leukocyte counting value to obtain a corrected reticulocyte counting value. By means of correction to the reticulocyte counting value by the leukocyte counting value, influences on count of the reticulocyte due to immature granulocyte or archaeocyte in the leukocyte is reduced and accuracy of the reticulocyte counting value is improved.

The invention provides a leukocyte classification counting method and device and a blood analyzer, and the method comprises the steps: obtaining an initial leukocyte histogram of leukocytes in a bloodsample, wherein the initial leukocyte histogram is a quantity and volume distribution histogram of leukocytes; defining a blood shadow area in the initial leukocyte histogram; and correcting the blood shadow region to obtain a corrected lymphocyte region. According to the leukocyte counting and classifying method, the leukocyte counting and classifying device and the blood analyzer provided by the embodiment of the invention, the interference of the blood shadow area to the lymphocyte area in the leukocyte histogram can be weakened and even eliminated, and the leukocyte counting and classifying accuracy is improved.

The invention belongs to the technical field of milk powder production, and particularly relates to a manufacturing and processing method for infant milk powder. The processing method comprises the main steps that the first step, inspection and measurement acceptance of original milk are conducted; the second step, purification of the original milk is conducted, specifically, high-speed centrifugal separation and purification of micro impurities such as somatic cells, white blood cells, red blood cells, dust and microorganisms in the impurities are conducted; the third step, cooling and storage are conducted, specifically, purified raw milk is cooled to 2-6 DEG C for storage; the forth step, preheat homogenization for sterilization is conducted, specifically, fat, added ingredients and thelike in milk are crushed; the fifth step, condense is conducted, specifically, the milk is vaporized at the temperature of 70-90 DEG C under vacuum pressure of -0.08-0.0895Mpa, and then an ultrafiltration membrane module is used for membrane separation; and the sixth step, drying and packaging are conducted. According to the manufacturing and processing method for the infant milk powder, the ultrafiltration membrane module is used for separating a membrane to prepare a milk powder wet material; and then spreading equipment is used for evenly spreading the milk powder wet material in a dryingplate, and thus the drying efficiency of wet milk powder can be accelerated.

This invention provides an abnormally-activated-cell apheresis-therapy apparatus for inhibiting the onset of or treating leukemia by removing abnormally activated cells, specifically abnormally activated leukocytes or leukemia progenitor cells, from blood. Said abnormally-activated-cell apheresis-therapy apparatus, which is an abnormally-activated-leukocyte apheresis-therapy apparatus that removes abnormally activated cells such as leukemia cells from blood, said cells having been made identifiable via high-concentration accumulation of protoporphyrin IX, is provided with the following: a blood-collection line, one end of which is provided with a needle for blood collection; a centrifugal separator that separates out a leukocyte fraction from blood sent down the blood-collection line; a cell sorter that removes abnormally activated cells from the separated-out leukocyte fraction; a light-exposure device that exposes the normal leukocyte fraction resulting from the removal of the aforementioned abnormally activated cells to light of a prescribed wavelength; and a return line that returns, to the patient, the normal leukocyte fraction and a return fluid comprising the blood components other than the leukocyte fraction.

The invention discloses an SOC-based leukocyte counting system. The system is characterized by comprising an SOC-FPGA, an SOC-ARM and a cache module, wherein an image collection module of the SOC-FPGAcollects a to-be-analyzed image from an image sensor in real time, and data of the to-be-analyzed image is preprocessed; the SOC-ARM comprises a DDR3 controller, is used for completing initializationconfiguration and dynamic configuration of the image sensor, data communication, image caching and display and software and hardware interaction time sequence control and is connected with the SOC-FPGA through an AMBA AXI4-Lite bus; and the cache module realizes caching of the data of the to-be-analyzed image by the adoption of a DMA data transmission IP core and is connected with the DDR3 controller through an AMBA AXI4 bus interface. Through the counting system and method, development cost can be lowered while image algorithm acceleration computing is realized quickly and efficiently.

The invention concerns a circulation vessel (300) adapted to an optical device (120) for leukocyte counting and / or differentiation in a blood analyzing automaton. The invention is characterized in that in an analyzing zone of the vessel (403), the cross section of the vessel has at least one transverse dimension ranging between 1 and 5 millimeters. It can advantageously be made, at least partly, from an injected plastic material and comprises at least one lens (302) molded in one single piece with the vessel (300).

The invention discloses a synchronous detection device combining immunodetection with leukocyte counting and a detection method thereof, and belongs to the technical field of analysis and detection. When the device provided by the invention is in use, immunological index detection and total leukocyte counting can be automatically and synchronously completed only by dropwise adding a detected bloodsample into the sample adding opening of a detection card, so the operation is simple, and the detection efficiency is high. The device provided by the invention is simple in structure and convenientto carry, and can be suitable for bedside detection, primary diagnosis and treatment and sick and wounded condition detection in a special environment. The detection method using the device is long in detection time window and is not interfered by impurities, so the detection accuracy is improved.

Chronic prostatitis (CP) is a syndrome characterized by pelvic pain in one or more areas (perineum, lower abdominal, testicular and / or penile), urinary frequency, urinary urgency, sexual dysfunction and / or ejaculatory pain. CP type IIIa is the most common type of prostatitis and is defined by having sterile EPS and a leukocyte count greater than 1000 / mm2. A method of prevention of chronic prostatitis is disclosed that comprises orally administering a composition containing a therapeutically effective amount of rifaximin to a patient exhibiting symptoms of chronic prostatitis.

The invention discloses a handheld leukocyte counter and a leukocyte counting method. The handheld leukocyte counter comprises a handheld shell, a photoelectric detection module, a main control unit,a man-machine interaction module, a detection card cabin door, a bracket, a detection card and the like, wherein the photoelectric detection module, the main control unit, the man-machine interactionmodule, the detection card cabin door, the bracket, the detection card and the like are assembled together, and the detection card is of a slit micro-fluidic chip structure and counts leukocytes according to the fluorescence excitation principle. The counter is small in size, light in weight and convenient to carry and place; the counting method can avoid interference of impurities; counting is accurate, precision is high, detection operation is easy, detection efficiency is high, and the counter is particularly suitable for leukocyte detection in primary medical treatment, mobile medical treatment and emergency rescue medical diagnosis in special environments.

A method for improving the accuracy of leukocyte classifying result of blood sample comprises: A, in the counting process of leukocyte, adding hemolytic agent in two times, testing and obtaining a first leukocyte counting number after adding the hemolytic agent at first time and a first leukocyte histogram, and a second leukocyte counting number after adding hemolytic agent at second time, while the hemolytic dosage added in the first time makes red blood cell fragments left affecting the counting number of leukocyte in the blood sample, and the hemolytic dosage added in the second time makesno red blood cell fragments left to avoid affecting the counting number of leukocyte; B, according to the ratio between the first leukocyte counting number and the second leukocyte counting number, determining a leukocyte classification histogram, and classifying leukocytes. The method can confirm the accuracy of leukocyte counting number and hemoglobin test result, and can improve the classification accuracy of leukocyte of animal blood samples of dog, cat and the like.

A Chinese medicine for promoting the regeneration of white cell and treating the leukocytopenia caused by chemicotherphy or radiotherapy is prepared from ginseng, atractylodes rhizome, rice vinegar and green alum through baking and pulverizing ginseng and atractylodes rhizome to obtain particles less than 120 meshes, mixing, calcining and pulverizing others to obtain particles less than 12 meshes, and mixing. Its advantages are high curative effect and no toxic by-effect.

The present disclosure relates to a prognostic method for assessing the risk of liver cancer in a patient with liver cirrhosis. The method comprises: collecting blood samples of the patient with liver cirrhosis; analyzing a set of clinical data, the clinical data including fasting blood glucose, insulin, high-density lipoprotein, platelets, lymphocytes and neutrophils; analyzing the set of clinical data calculating a risk index; and making predictions based on the risk index. According to the risk index, the risk of liver cancer of the cirrhotic patient can be accurately and efficiently predicted, so that he can receive appropriate treatment in time.

The invention belongs to the technical field of preparation of molecular markers of domestic animals and particularly relates to a molecular marker related to a porcine leukocyte count, and application of the molecular marker to marker-assisted selection of pigs. The molecular marker is a fragment of an SN gene; a nucleotide sequence of mRNA of the molecular marker is shown as a sequence table SEQ ID NO: 1; a mutant allele exists on a 699 bp site of the sequence table, namely a 367G-367A mutant exists on a 367bp site of a sequence shown as figure 4, which results in Hin6I-RFLP polymorphism. The invention further discloses a primer for amplifying the nucleotide sequence of the SN gene and a polymorphism detection method. The invention further discloses application of the prepared molecularmarker to correlation analysis on the porcine leukocyte count; and a new molecular marker is provided for the marker-assisted selection of the pigs.

The invention relates to a system for predicting the risk of death of a patient. The system for predicting the risk of death of a patient comprises: a data acquisition module used for obtaining the medical history of a patient, physiological parameters of the patient and laboratory parameters of the patient, wherein the physiological parameters comprise the Glasgow Coma Scale of the patient, the heart rate of the patient, the systolic pressure of the patient and the oxygen saturation of the patient, and the laboratory parameters comprise the hemoglobin level, the leukocyte count, the creatinine concentration, the blood potassium concentration, the blood sodium concentration, the urea content, the platelet count, the total bilirubin concentration, the D-dimer level and the fibrinogen content of the patient; and a module for calculating the death risk of the patient used for calculating the information acquired in the data acquisition module so as to calculate the 7-day death rate (p) ofthe patient.

The invention discloses a leukocyte counter and a leukocyte counting method. The leukocyte counter comprises a human-computer interaction module, a photoelectric detection module, a main control unitand a detection card, wherein the detection card is arranged in the photoelectric detection module, and the main control unit is electrically connected with the man-machine interaction module and thephotoelectric detection module. The leukocyte counter has the characteristics that (1) the leukocyte counter is small in size, light in weight and convenient to carry and place; (2) a fluorescence excitation method is adopted as a working principle, so the interference of impurities is avoided, counting is accurate, and precision is high; (3) the operation is simple during detection, and the detection efficiency is high; and the deficiency of leukocyte detection instruments in existing primary medical treatment, mobile medical treatment and emergency rescue medical diagnosis in special environments can be made up.

The invention relates to a preparation method of a subacid carbonic acid type hypochlorous acid water disinfectant. The preparation method specifically comprises the following steps: 1) preparing a hypochlorous acid stock solution A, a citric acid stock solution B, a vitamin C stock solution C, a carbonic acid type subacid buffer stock solution D and a sucrose stock solution E; 2) preparing subacid carbonic acid type hypochlorous acid disinfection and sterilization water; and 3) conducting filtering and packaging. The subacid carbonic acid type hypochlorous acid water disinfectant has the advantages that the prepared subacid carbonic acid type hypochlorous acid water disinfectant is stable in subacidity which is matched with the subacidity of human physique, the disinfection and sterilization principle of hypochlorous acid generated by human leukocyte is perfectly simulated, and the subacid carbonic acid type hypochlorous acid water disinfectant has very high sterilization capacity andis non-toxic and harmless to human bodies.

A method for counting leukocytes which comprises liberating elastase from granulocytes contained in a specimen, adding an anti-granulocyte elastase antibody to the thus liberated elastase, measuring the antibody bonded to the elastase to thereby determine the concentration of the elastase, and then calculating therefrom the number of leukocytes contained in the specimen with the use of the known ratio of the leukocyte count to the elastase concentration. This method makes it possible to conveniently and less expensively count leukocytes at a high accuracy comparable to the one established by using a conventional automatic blood cell counter, as the figure shows.

The invention discloses a growth factor extraction device. The growth factor extraction device comprises a centrifugal machine, an inflation mechanism and a plurality of centrifugal units, a plurality of mounting holes are formed in a centrifugal table of the centrifugal machine, and an inflation connector is arranged at the bottom of each mounting hole. The inflating mechanism is used for inflating each inflating connector; the centrifugal unit comprises a lower cylindrical body and an upper cylindrical body; a centrifugal cavity is formed in the lower cylindrical body, a piston is arranged in the centrifugal cavity in a matched mode, a connector is coaxially arranged at the lower end of the lower cylindrical body, and the lower end of the lower cylindrical body is inserted into a mounting hole and is in threaded connection with the inflation connector through the connector; the lower end of the upper cylindrical body is detachably and rotatably connected with the upper end of the lower cylindrical body, and a leucocyte cavity, a platelet cavity and an erythrocyte cavity are arranged in the upper cylindrical body. According to the device, red blood cells, white blood cells and platelets can be automatically separated after blood is centrifuged and layered, the separation difficulty is reduced, the separation efficiency is improved, separation is more thorough, and platelet gel is not prone to being damaged in the separation process.

The invention relates to a system for evaluating prognosis of a patient with spinal glioma based on peripheral blood cells. The system is characterized by comprising an input device, a computing device and an output device. The input device is used for inputting a plurality of peripheral blood cell counting units of an individual spinal glioma patient, and the plurality of peripheral blood cell counting units comprise a leukocyte counting unit, a neutrophil counting unit, a platelet counting unit and a lymphocyte counting unit. Through the above technical scheme, peripheral blood of a patientwith spinal glioma only needs to be collected, and the peripheral blood cell counting number in the peripheral blood is detected; the prognosis score of the patient can be calculated and output according to various blood cell counting numbers; the prognosis condition of the patient can be evaluated according to the prognosis score; the peripheral blood collection process is simple and rapid, and the detection operation complexity of the blood cell count in the peripheral blood is low, such that the prognosis condition of the spinal glioma patient can be simply, rapidly and accurately evaluated.

Provided according to the present invention is a method for assessing immunity of peripheral blood and providing information on whether or not the onset of colorectal cancer has begun by utilizing a difference in immune cell distribution between peripheral blood of colorectal cancer patients and normal persons, the method comprising the steps of: (A) analyzing peripheral blood immune cells for cell size and wrinkle degree to classify the cells into lymphocytes, monocytes, and granulocytes; (B) staining a labeling marker of the three kinds of immune cells with at least one antibody combinationto analyze the distribution of immune cells in peripheral bloods of cancer patients and normal persons; (C) discriminating sets in which meaningful result values of the labeling marker are different between the two groups, cancer patients and normal persons, with statistical significance, so as to discriminate whether or not the onset of cancer has begun among cancer patients and normal persons; and (D) measuring immunity per unit of blood by means of the labeling marker to diagnose whether or not the onset of colorectal cancer has begun, without counting natural killer cells with a flow cytometer or a cell counter.

The invention discloses a traditional Chinese medicine composition for novel coronavirus pneumonia complications and a preparation method thereof. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 10-30 parts of herba lysimachiae christinae, 30-50 parts of rhizoma drynariae, 50-70 parts of radix cynanchi paniculati, 30-50 parts of cortex ilicisrotundae and 10-30 parts of caulis trachelospermi. The preparation method comprises the following steps: step I, weighing the raw materials in parts by weight; step II, cleaning and drying the weighedraw materials, and performing mixing to obtain a raw material mixture; step III, crushing the raw material mixture, and sieving the crushed raw material mixture with a 60-80-mesh sieve to obtain a crushed material; and step IV, packaging the sieved crushed materials with 30g in each bag. The traditional Chinese medicine composition disclosed by the invention is simple to prepare, low in price, good in curative effect and free of toxic and side effects, can be taken after being mixed with boiled water and decocted with water, gives full play to the curative effect, can effectively remove viruses, accelerates elimination of fever, quickly relieves various symptoms of new coronal pneumonia, such as anhelation, dry cough, headache and arthralgia and muscle pain, and meanwhile, enhances the resistance of a human body, increases the leukocyte index, and enhances the anti-inflammation effect.

The purpose of the present invention is to provide an adsorption material capable of selectively adsorbing immunosuppressive leukocytes. The present disclosure provides an immunosuppressive leukocyteadsorption material containing a water-insoluble carrier to which is bonded one or more nitrogen-containing compounds selected from polyamines represented by a prescribed formula and aliphatic aminesrepresented by a prescribed formula, the water-insoluble carrier having the form of fibers or particles, the diameter of the fibers or the particles being 15-50 mum, and the arithmetic mean roughnessof the surface of the water-insoluble carrier being 0.1-3.0 mum.

A method for counting leukocytes which comprises liberating elastase from granulocytes contained in a specimen, adding an anti-granulocyte elastase antibody to the thus liberated elastase, measuring the antibody bonded to the elastase to thereby determine the concentration of the elastase, and then calculating therefrom the number of leukocytes contained in the specimen with the use of the ratio of the leukocyte count to the elastase concentration. This method makes it possible to conveniently and less expensively count leukocytes at a high accuracy comparable to the one established by using a conventional automatic blood cell counter, as the figure shows.