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Post extraction production process of pullulanose

A pullulan sugar and production process technology, applied in the directions of fermentation, ultrafiltration, etc., can solve the problems of inflammable, explosive, unsafe, large steam consumption, etc., and achieve high extraction yield, good quality and high product yield Effect

Inactive Publication Date: 2006-12-13
山东中清生物科技有限公司
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  • Description
  • Claims
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Problems solved by technology

At present, the post-extraction production process of polysaccharides (such as xanthan gum, thermosetting polysaccharide, gellan gum) uses organic solvents such as alcohol and isopropanol as dehydrating agents to dehydrate the polysaccharide fermentation broth, and simultaneously decolorize and desalt the product. Then dry, because a large amount of organic solvents are used in the production process, so the distillation recovery of organic solvents needs to consume a large amount of steam, and because these organic solvents are flammable and explosive, there are great unsafe factors in the production process

Method used

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  • Post extraction production process of pullulanose

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Embodiment Construction

[0015] Post-extraction production process of pullulan: as shown in the figure.

[0016] 1. The pullulan fermentation liquid is filtered to remove bacteria, and the clear liquid is pumped into the filtrate storage tank.

[0017] 2, the clarification pullulan sugar fermented liquid (7m 3 ) into the hollow fiber ultrafiltration membrane separator, the molecular weight cut-off of the membrane is 6000D, the retained liquid is returned to the storage tank, and water, pigment and inorganic salt permeate the membrane. During this process, deionized water is continuously added to the storage tank to remove The additional amount of deionized water is 50% of the volume of the permeate. When the chroma and salinity reach the standard, stop adding deionized water; continue the ultrafiltration operation until the concentration reaches 20%.

[0018] 3. The concentrated pullulan solution is pumped into the fluidized bed, and dried to obtain a 430Kg sample. The extraction yield is 97.8%.

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Abstract

A post-extracting process for pullulanose features that after the bacteria have been removed, the fermented liquid of pullulanose is decolored, desalted and concentrated by membrane filtering technique, and then directly dried by fluidized-bed drier. Its advantage is high extraction rate.

Description

technical field [0001] The invention relates to the industrialized production technology of pullulan, in particular to a post-extraction production technology of pullulan. Background technique [0002] Pullulan is a microbial exopolysaccharide with high application value. It is a linear polysaccharide formed by polymerizing maltotriose repeating units linked by α-1,4-glycosidic bonds through α-1,6-glycosidic bonds. . However, due to the different strains used, the maltotriose unit in the polysaccharide chain is occasionally replaced by a very small amount of maltotetraose, maltose or glucose residues, and branched chain structures also appear in individual cases. The molecular weight of pullulan is generally 5.0×10 4 -5.0×10 6 Between daltons; its molecular weight is greatly affected by the culture conditions. Controlling the fermentation conditions can fix its molecular weight in a certain range. The research on pullulan started in West Germany, and the British also di...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C12P19/10B01D61/14C12P19/04
Inventor 苏理兰文忠赵双枝董学前
Owner 山东中清生物科技有限公司