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Novel gene P40 containing acyl group carrying protein-like structural domain

A domain and gene technology, applied in the field of molecular biology, can solve the problem of not containing acyl-carrying proteins and so on

Inactive Publication Date: 2006-12-20
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are only more than 20 tumor suppressor genes and tumor suppressor candidate genes that have been discovered so far, and there is no gene containing an acyl-carrying protein (ACP)-like domain among known genes

Method used

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Examples

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Embodiment Construction

[0114] Embodiment of the invention

[0115] Taking transfection of BT325 cells and rheumatoid arthritis synovial cells as an example, 1 ug of DNA plasmid containing p40 gene was transferred into about 10 subcultured cells by liposome-mediated method. 6 BT325 cells or rheumatoid arthritis synovial cells, after 48 hours, the cell cycle was detected by flow cytometry, and 49.1% of the cells in the p40 genome were in the G1 (before DNA synthesis) phase, and 30.5% of the cells were in the S (DNA synthesis) phase. ) phase, while only 39.7% of the cells in the empty vector control group were in the G1 phase, and 41.7% of the cells were in the S phase. 2.8-6.3% of rheumatoid arthritis synoviocytes transfected with p40 gene were apoptotic.

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Abstract

The present invention relates to molecular biology. Up to now, only twenty odd kinds of cancer inhibiting genes have been obtained and they have no the characteristic of containing the structural domain similar to acyl group carrying protein. The novel gene of the present invention, p40, has cDNA whole length of 2024 bp and complete reading frame, encodes protein containing 357 amino acid residues of molecular weight 40 kD, is one difference expressing gene for the normal brain tissue high expression, glioma tissue and glioma cell low expression or no expression, and is one very good cancer inhibiting gene capable of inhibiting the growth of glioma cell.

Description

Technical field: [0001] The present invention relates to molecular biology techniques Background technique: [0002] It is estimated that there are 100,000 different genes in the human genome, of which only 9,000 are known, and most of the genes and their functions are unknown. Tumor is the second leading cause of death in human beings, which seriously threatens human health and life. The mechanism of tumorigenesis and development is still not fully understood. However, it has been recognized that tumors are the product of activation of proto-oncogenes and / or deletion or inactivation of tumor suppressor genes. Therefore, on the road to overcoming cancer, scientists are devoting themselves to the research of tumor suppressor genes, hoping that by introducing certain tumor suppressor genes into tumor cells, the growth and proliferation of cells can reach a new balance. There are only more than 20 tumor suppressor genes and tumor suppressor candidate genes that have been dis...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12N15/10
Inventor 邓艳春药立波刘新平王吉村韩华王国华
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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