Method for preparing chitosan/chitose in molecular weight narrow distributed
A technology of narrow molecular weight distribution and chitosan oligosaccharide is applied in the field of preparing narrow molecular weight distribution chitosan oligosaccharide, which can solve the problems of difficult control and difficulty in the degradation process, and achieve the effects of saving raw materials, simple production equipment and simple method.
Inactive Publication Date: 2007-08-22
HAINAN UNIV
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AI Technical Summary
Problems solved by technology
Both acid hydrolysis and simple oxidative degradation are non-specific degradation processes, and the degradation process is difficult to control. Although it has been used in industrial production successively, and chitosan products in various molecular weight ranges can be obtained, but if you want to obtain a specific molecular weight It is more difficult to produce chitosan products with high yield and wide application; other degradation methods such as microwave method, ultrasonic method, radiation method, etc., can also obtain oligomeric water-soluble chitosan, but they are still non-specific The degradation process is still in the stage of basic research and exploration
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0024] In order to further illustrate the principle, process and results of the PACu-CMPS template adsorption-controlled length / catalytic oxidation cracking method of the present invention, examples are now described in detail.
[0025] Weigh 50 grams of chitosan and put it into a reaction kettle with a stirrer, add 4000cm 3 0.5% acetic acid, stirred for 60 minutes to dissolve chitosan. Weigh 5 grams of ground and sieved 100-mesh PACu-CMPS, wet it with water, add it to the reaction kettle, and stir evenly. with 5% Na 2 CO 3 Adjust the pH to 6.5. Add 100cm 330% hydrogen peroxide, stirred at room temperature for 80 minutes, heated to 65°C (±1°C) for 4 hours. Add 1000cm under stirring 3 0.05 acetic acid aqueous solution, suction filtered and washed 3 times, and the filtrate was kept at room temperature or frozen to dryness. HAc-NH for products 4 Dissolve in Ac (pH=4.5) buffer, and use HAc-NH 4 Ac was used as the mobile phase, and water-soluble dextran standard samples wi...
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Abstract
The invention relates to a method for preparing chitosan oligosaccharide with narrow molecular weight distribution. Said invention adopts the template coordination to control length and catalytic oxicracking mode to prepare the chitosan oligosaccharide with narrow molecular weight distribution. Its techanical scheme uses edible acetic acid to dissolve high-polymer chitosan solid to obtain solution, and includes the following steps: making the chemically-synthetic and pulverized-mesh screened coper p-dibenzoate (II) solid metal be coordinated with high polymer granule PACu-CMPS microparticle, stirring them and adding them into the above-mentioned solution, regulating pH value to make amino-group in chitosan and bare metal ions on the PACu-CMPS granule surface have weak coordination bond formation, adding oxidant at room temp. andmixing them uniformly, heating and degrading, filtering and separating out degradation liquor, low-temp. or room temp. concentrating so as to obtain the solid powder product.
Description
technical field [0001] The invention relates to a method for preparing chitosan oligosaccharide with narrow molecular weight distribution. Background technique [0002] The present invention relates to a kind of using the amino group in chitosan and chemically synthesized teredibenzoate copper (II) solid metal coordination polymer particles (abbreviation: PACu-CMPS) surface exposed metal ions to combine through weak coordination bonds, A method for preparing oligosaccharides rich in narrow molecular weight distribution by adding an oxidant to catalyze oxidative degradation, filtration, and other post-treatments. More specifically, chemically synthesized teredibenzoate copper (II) solid metal coordination polymer particles are used as a template adsorption matrix for adsorbing chitosan, and are pulverized to a certain particle size. Add the weakly acidic and insoluble PACu-CMPS into the chitosan aqueous solution after deacetylation of biological chitin dissolved in 0.5% acet...
Claims
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IPC IPC(8): C08B37/08
Inventor 张岐王爱民屈淑玉
Owner HAINAN UNIV