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Composition for regulation of gametophytic self-incompatibility, control method of gametophytic self imcompatibility of a plant and the plant self-polli-nated by using said control method

A compound and gametophyte technology, which are applied to the compound regulating gametophyte self-incompatibility, regulating the gametophyte self-incompatibility of plants and using the above-mentioned regulation and self-pollination in the field of plants

Inactive Publication Date: 2002-08-14
ISTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, many researchers in the world are now studying an inhibitor of pollen or a receptor molecule, but they have no clear clues

Method used

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  • Composition for regulation of gametophytic self-incompatibility, control method of gametophytic self imcompatibility of a plant and the plant self-polli-nated by using said control method
  • Composition for regulation of gametophytic self-incompatibility, control method of gametophytic self imcompatibility of a plant and the plant self-polli-nated by using said control method
  • Composition for regulation of gametophytic self-incompatibility, control method of gametophytic self imcompatibility of a plant and the plant self-polli-nated by using said control method

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0064] Isolation and purification of column-specific RNase in Example 1, a regulatory protein of gametocyte self-incompatibility Isolation and purification of RNase from each organ of Fuji apple

[0065] Isolate RNase from the stem, root, leaf, handle, petal and calyx of Fuji apple, the experimental steps are as follows:

[0066] Add 10mM Na 2 PO 4 (pH 6.0), 10 mM EDTA, 1 mM PMSF and 1% polyvinylpyrrolidine buffer solution, mix them uniformly in a mortar, and extract the total protein. Utilize 40% Na 2 PO 4 Concentrate the proposed total protein, then dialyze the concentrated total protein to 5 mM Na using a semi-permeable membrane (molecular cutoff: 12,000 Dalton 2 PO 4 in the buffer solution. A fraction with high RNase activity was finally obtained, with a total RNase activity of 295,000 units.

[0067]Using a resin column (Bio-Rad, England) filled with Biogel P-60, the fraction with high RNase activity was subjected to gel filtration chromatography (Harris et al., P...

example 2

[0085] Inhibition of Pollen Tube Elongation in Self-Pollen by Stem-Specific RNase of Fuji Apple

[0086] In order to study the role of the purified, stele-specific RNases in Examples 1-4 on the elongation of pollen tubes of self-pollen, we conducted the following experiments. First, the self-pollen in the male stamens of the Fuji apple is isolated. The isolated pollen was cultured in a medium for pollen tube elongation, and 0, 2, 4 and 6 units (mg -1 min -1 ml), the S1 RNase purified in Example 1-4 was incubated in the dark for 24 hours. The medium for pollen tube elongation contained 20 mM Mes-KOH (pH 6.0), 0.07% Ca(NO 3 ) 2 4H 2 O, 0.02% MgSO 4 ·7H 2 O, 0.01% KNO 3 , 0.01%H 3 BO 3 And 2% sucrose, can artificially prolong the pollen tube.

[0087] For laboratory results of the pollen tube elongation pattern see image 3 . Such as image 3 As shown in , self-pollen pollen tube elongation was inhibited. From this result, it was confirmed that the decisive factor...

example 3

[0089] Study on Inhibitors of Stem Specific RNase

[0090] The difference in activity of the purified stem-specific S1 RNase and S2 RNase in Examples 1-4 can be studied by adding chemicals that act as inhibitors of proteolytic enzymes. By adding common RNase activity agonists, adding commercially available RNase activity inhibitors and adding the inhibitor of the column-specific RNase just invented at present, the activity of RNase can be detected (Singh A. et al., Plant Physiology , 96:61-68, 1991). Specifically, 1 mM EDTA, a representative inhibitor of proteolytic enzymes; 1 mM calcium chloride, an activator of RNase activity; 1 mM DEPC (Sigma), a commercially available lethal toxic Inhibitors of RNase activity; and 1mM ZnSO 4 and 1mM CuSO 4 , that is, the column-specific RNase inhibitor to detect RNase activity. See the experimental results Figure 4 .

[0091] The result is: compared with the control group with 100% RNase activity in phosphate buffer, when adding 1mM...

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Abstract

The present invention relates to a regulation composition for gametophytic self-incompatibility which contains sulfates especially CuSO4 and ZnSO4 as an inhibitor, which prevents a style-specific RNA enzyme activity regulating gametophytic self-incompatibility; a control method for gametophytic self-incompatibility of a plant by using the regulation composition for gametophytic self-incompatibility; and a plant self-pollinated by destroying the gametophytic self-incompatibility, using the control method. By using the regulation composition for gametophytic self-incompatibility according to the present invention, a single species of fruit trees can be self-pollinated without cultivating other pollinizer, so that fruition rate can be increased and the productivity per unit area can be maximized.

Description

technical field [0001] The invention relates to a regulating compound for gametophyte self-incompatibility, a method for regulating gametophyte self-incompatibility and self-pollination plants using the regulation method. In particular, the present invention relates to a modulatory compound for gametocyte self-incompatibility comprising sulfate, especially CuSO 4 and ZnSO 4 As an inhibitor, it can prevent the activity of a stele-specific RNA enzyme that can regulate the self-incompatibility of the gametophyte; a method for regulating the self-incompatibility of the plant gametophyte is through This is achieved using a gametophyte self-incompatibility modulating compound; and a plant self-pollinates by disrupting gametophyte self-incompatibility using the above-mentioned modulating method. Background technique [0002] The flowers of more than half of the plants in the world have self-incompatibility of gametophytes. The self-incompatibility of the gametophytes means that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H3/04A01N57/16A01N59/06A01N59/16A01N59/20
CPCA01H1/02A01N59/16A01N57/16A01N59/06A01H3/04A01N59/20A01H1/026A01H1/022A01N25/30A01N2300/00
Inventor 郑镒炅柳宗相金明姬许炳柱郑镒仙
Owner ISTECH CO LTD
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