Skin care composition
A composition and skin technology, applied in the directions of skin care preparations, cosmetics, cosmetic preparations, etc., can solve the problems such as the special synergistic effect of coupling linoleic acid and retinoic acid that have not been disclosed, and achieve a wide range of skin care benefits, improvement Effects of improved skin color, texture and condition
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Embodiment 1
[0069] This example illustrates the synthesis of coupled linoleic acid containing the c9 t11 isomer and the t10 c12 isomer. Preparation of Mixed Isomers of CLA
[0070] 'Analar's reagent' (AR) sodium hydroxide (0.6 kg) was dissolved in 6 kg of pharmaceutical grade propylene glycol by mixing and heating at 80-85°C. The sample was allowed to cool and 2 kg of safflower oil was added. The mixture was refluxed at 170°C for 3 hours with rapid stirring using standard small-scale equipment. The reaction mixture was cooled to about 95°C, the stirrer was reduced to medium speed, and the mixture was neutralized with 1.280 L of 35.5% hydrochloric acid dissolved in demineralized water (8 L), maintaining the temperature at about 90°C. The reaction mixture was allowed to stand and the aqueous phase was removed. The oily phase was washed with 2 x 1 L of 5% AR salt emulsion and 2 x 1 L of demineralized water at 90 °C, discarding any soapy material. The CLA-enriched oil was dried under vacu...
Embodiment 4
[0101] Example 4 Identification of Procollagen-I and Decorin Upregulation in Skin in Vivo for Comparative Purposes Following Topical Retinoic Acid Treatment
[0102] Collagen is the major matrix skin protein known to impart tensile strength to the skin. Decorin is a proteoglycan important for controlling and correcting collagen deposition in the extracellular matrix of the skin. It is also known that the levels of collagen and decorin in skin are significantly reduced in aged and / or photodamaged skin. Many studies have shown that the level of collagen type I in the skin decreases with age and / or with increased photodamage, (e.g. Lavker, R.J.Inv.Derm., (1979), 73, 79-66; Griffiths et al. Eng. J. med. (1993) 329, 530-535). In the case of decorin, mRNA expression and proteoglycan expression have been shown to be significantly reduced in photodamaged skin in vitro (Bernstein et al. Lab. Invest. (1995) 72, 662-669). Thus, decreased levels of these skin proteins are associated with...
Embodiment 5
[0112] Example 5 Measurement of Procollagen-I and Decorin Synthesis in Human Skin Fibroblasts Preparation of Conditioned Medium for Skin Fibroblasts
[0113] Phase 2 (P2) primary human foreskin fibroblasts were seeded in 12-well plates at 10,000 cells / cm 2 and maintained for 24 hours in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum in an atmosphere of 5% carbon dioxide and 4% oxygen. Thereafter, cells were washed with serum-free DMEM and incubated in fresh serum-free DMEM for 60 minutes. The fibroblast monolayer was then washed again with serum-free DMEM. Assay reagents and vehicle controls were added to cells in triplicate to a final volume of 0.4 ml / well fresh serum-free DMEM and further incubated for 24 hours. This fibroblast conditioned medium was either assayed immediately or snap frozen in liquid nitrogen and stored at -70°C for further analysis. Thereafter cells were counted and data from dot blot analysis were normalized to cell nu...
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