Method of eliminating human serum albumin polymers
A technology of human serum albumin and albumin, applied in the direction of serum albumin, albumin peptide, peptide preparation method, etc., can solve the problems of low productivity and so on
Inactive Publication Date: 2010-05-26
KM生物医药股份公司
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Problems solved by technology
[0008] When multiple purification methods are used in combination, the cumulative multiplication of the recoveries of the individual steps often results in low productivity
Method used
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Embodiment 1
[0032] Example 1: Strong Anion Exchange Chromatography Following Dialysis
[0033] The human serum albumin solution that the human serum albumin multimer content prepared according to the method for preparation example is 6.1% (according to the quantitative value that gel filtration HPLC analyzes obtains) in the sodium chloride containing 50mM, 75mM or 100mM respectively In 50mM sodium phosphate buffer (pH6.5), after dialysis at room temperature for 8 hours, the human serum albumin solution was added to the Q-Sepharose FF column (diameter 2.5cm x 10cm) equilibrated with the same buffer in advance, Elution was performed with the same buffer solution (500ml, elution rate: 12ml / min), and all the effluent components were recovered.
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A method of eliminating human serum albumin polymers characterized by bringing a human serum albumin solution containing human serum albumin polymers into contact with an anion exchanger having been equilibrated with a buffer solution which has a salt concentration of 10 to 150 mM and a pH value of 5 to 9.5.
Description
technical field [0001] The present invention relates to a method for removing human serum albumin multimers. Specifically, it refers to a method for removing human serum albumin multimers by contacting a human serum albumin solution containing human serum albumin multimers with an anion exchanger under specific conditions. Background technique [0002] Human serum albumin (hereinafter referred to as "HSA") is the main component in plasma, consisting of a single-chain polypeptide of 585 amino acids, with a molecular weight of about 66,000 Daltons (Minghetti, P.P.etal (1986) Molecular structure of the human albumin gene is revealed by nucleotide sequence within 11-22 of chromosome 4. J. Biol. Chem. 261, 6747-6757). It is known that the main function of HSA is to maintain the normal osmotic pressure of the blood and combine various substances such as calcium ions, fatty acids, bilirubin, tryptophan and drugs in the blood, and play a role as a carrier in the transport of these ...
Claims
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IPC IPC(8): C07K1/18C07K14/765C12P21/02A61K38/00A61K35/16A61P7/08C07K1/14
CPCC07K14/765C07K1/18A61P7/08
Inventor 足达聪沟上宽田嶋义高宫津嘉信野内俊伸牛尾义高
Owner KM生物医药股份公司