Biomimetic biological reaction device of cells utilized for screening medication

A reaction device and cell biology technology, which is applied in the field of bionic cell biological reaction devices, can solve the problems of prolonging the cell culture cycle, high pollution rate, and heavy workload, and achieve the goal of prolonging the cell culture cycle, facilitating sampling and analysis, and realizing automatic management Effect

Inactive Publication Date: 2003-10-08
TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose and task of the present invention is to provide a bionic cell biological reaction device for drug screening, to overcome the defects of traditional square bottle culture cells that are easy to cause false positives and complicated procedures, heavy workload, and high pollution rate; it can ensure regular replacement Cell culture medium, add the sample to be screened at any time of the cell culture cycle, prolong the cell culture cycle, and at the same time realize the automatic management of the culture process

Method used

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  • Biomimetic biological reaction device of cells utilized for screening medication
  • Biomimetic biological reaction device of cells utilized for screening medication
  • Biomimetic biological reaction device of cells utilized for screening medication

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1 (cell culture)

[0032] use Figure 5 For the device shown, first connect the system components, including the gas supply channel, culture solution storage bottle, cell proliferation square bottle, buffer bottle and waste liquid bottle, and check the airtightness according to the process requirements, and then place them in a high-pressure steam cooker for general use. Method for sterilization. After sterilizing and cooling to no higher than 37°C, put 500ml of the previously filtered and sterilized culture solution into the culture solution storage bottle in the aseptic operation table, and insert cells into the cell proliferation square bottle, and the inoculation density is about 5× 10 4 pcs / ml, the initial liquid volume in the bottle is about 20ml. After that, place the cell proliferation flask in a water bath with the temperature set at 37°C, start the air supply system for the cell proliferation flask, and maintain it statically for 12 hours, then p...

Embodiment 2

[0033] Embodiment 2 (norcantharidin (Nc) is to the action effect of cell in the cell proliferation square bottle)

[0034] use Figure 5 In the device shown, the drug concentration of Nc used is 10 μg / ml, and the cells used are SH-SY5Y. The action mode of Nc on the cells in the cell proliferation flask was cycle action mode. Observed after 72 hours, the experimental results are as follows Figure 10 The data are the average of 4 experiments. In the figure, the abscissa of the cell proliferation bottle CPB8, the cell proliferation bottle CPB12, the cell proliferation bottle CPB24 and the cell proliferation bottle ∞ represents the operation of administration every 8 hours, 12 hours, 24 hours and ∞ hours. Among them, ∞ hours means that the interval is infinitely long, which is actually the mode of action in the ordinary square bottle. In the actual experiment, it is not necessary to experiment on the cell proliferation square bottle, and a multi-well plate can be used instead....

Embodiment 3

[0037] Embodiment 3 (sophoridine (Sp) is to the dynamic effect of live cell)

[0038] use Figure 5 In the device shown, sophoridine (Sp) is used as the experimental drug, and Sy5y, Hela and HepG2 are used as the administration objects. In the experiment, the mode of action of Sp on the cells in the cell proliferation flask is circulatory. Compared with ordinary square bottles, this method is unique to cell proliferation square bottles. Taking the static effect of Sp on the cells in the cell proliferation square bottle and the effect on the cells in the common square bottle as a contrast, the experimental results are as follows: Figure 12 , 13 and 14 are shown. The common law is that during the static action process of the ordinary square bottle and the cell proliferation square bottle, and in the initial stage of the cell proliferation square bottle circulation state, the concentration of Sp no matter which kind of cells it interacts with will decrease, and in the same env...

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Abstract

A bionic cell bioreactor for screening medicines is composed of square bottle for reproducing cells, culture liquid bottle, buffer bottle, waste liquid bottle, sampling valve, computer, creep pump, electronic valve and connection pipeline. It can periodically exchange the nutritive liquid in square bottle, continuously apply medicine, culturing cells by high density, easily sample and analyze, and simulate the blood flow in blood vessel and creep of intestine.

Description

technical field [0001] The invention relates to a bionic cell biological reaction device for drug screening, which belongs to the field of bioengineering. Background technique [0002] Drug screening by culturing cells in square flasks is one of the general drug screening methods. The in vitro drug screening process of culturing cells in traditional square flasks requires aseptic operation of adding drugs again during cell inoculation or after cell culture for a certain period of time. The former is easy to cause false positives, while the latter has cumbersome procedures, heavy workload and high pollution rate. In addition, the in vitro growth environment of cells provided by the traditional square bottle is not easy to control, and usually changes with the culture process; in addition, this culture method cannot replenish the nutrients needed for cell growth at any time, so this method often leads to false positives and false negatives in research results pseudo-result p...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12Q1/18
CPCC12M29/18C12M41/48
Inventor 罗国安王义明肖炘肖盛元梁宁
Owner TSINGHUA UNIV
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