Method of increasing super rice yield applying transgene technique
A transgenic and super technology, applied in the fields of application, horticultural methods, rice cultivation, etc., can solve the problems that affect the high-yield goal of rice, the accompanying rice grains are insufficiently filled, and the fruit is not full.
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[0019] 1. Cloning of mutant starch synthesis key enzyme gene (AGP)
[0020] Mutagenesis Escherichia coli to produce AGP gene mutation, clone it and construct it on the transformation plasmid (PCAMB1A1303) of Agrobacterium to form two transformation plasmids, PT0129-1303 (17.4kb) and PT0125-1303 (20.1kb), and transform the two transformation plasmids into into Agrobacterium.
[0021] 2. Transformation of rice with Agrobacterium-mediated transformation of the plasmid
[0022] Select super rice varieties with insufficient grain filling or premature senescence, such as Zhongchao 123 and other varieties, which have significant heterosis, and the plants grow well, but the grains are not full. The immature embryos of Zhongchao 123 variety were induced into callus tissue, and the mutant AGP gene was introduced into the callus tissue according to the procedure of Agrobacterium-mediated transformation method. On the selection medium containing 50 mg / L hygromycin, the callus was screene...
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