Method of increasing super rice yield applying transgene technique

A transgenic and super technology, applied in the fields of application, horticultural methods, rice cultivation, etc., can solve the problems that affect the high-yield goal of rice, the accompanying rice grains are insufficiently filled, and the fruit is not full.

Inactive Publication Date: 2003-11-12
CHINA NAT RICE RES INST
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Problems solved by technology

[0004] At present, the problem existing in the research of super rice is that while utilizing inter-subspecies hybridization advantage, the grain filling of the associated rice is insufficient, the filling degree is low, that is, the grain is not full, and the plant appears premature senility, which seriously affects the realization of the high-yield goal of rice; the

Method used

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Embodiment Construction

[0019] 1. Cloning of mutant starch synthesis key enzyme gene (AGP)

[0020] Mutagenesis Escherichia coli to produce AGP gene mutation, clone it and construct it on the transformation plasmid (PCAMB1A1303) of Agrobacterium to form two transformation plasmids, PT0129-1303 (17.4kb) and PT0125-1303 (20.1kb), and transform the two transformation plasmids into into Agrobacterium.

[0021] 2. Transformation of rice with Agrobacterium-mediated transformation of the plasmid

[0022] Select super rice varieties with insufficient grain filling or premature senescence, such as Zhongchao 123 and other varieties, which have significant heterosis, and the plants grow well, but the grains are not full. The immature embryos of Zhongchao 123 variety were induced into callus tissue, and the mutant AGP gene was introduced into the callus tissue according to the procedure of Agrobacterium-mediated transformation method. On the selection medium containing 50 mg / L hygromycin, the callus was screene...

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Abstract

The present invention relates to a method for raising filling efficiency of rice seed, and a method of raising super-grade rich yield by using transgenic technique of plump rice seed. Said method includes the following steps: introducing the starch synthetic key enzyme gene (AGG) mutated by induction into the callus of immature embryo of super-grade rice variety of sparing grain, screening and cultivating to obtain rice seedling, transplanting it to greenhouse or field to obtain To generation plant, making agronomical character investigation of To generation plant in which the mutant AGP gene is transferred, selecting the seed of plant whose thousand-grain weight is relatively high and cultivating it to obtain Ti generation plant, and making agronomical character investigation of Ti generation plant.

Description

technical field [0001] The invention relates to a method for cultivating rice varieties, in particular to a method for improving rice yield by applying transgenic technology. Background technique [0002] my country's arable land accounts for 7% of the world's total, but it feeds 23% of the world's population. This is mainly due to the development of science and technology, which has enabled my country's rice harvests to increase year after year and continue to increase. The increase of rice production depends on the improvement of rice varieties. In the past 100 years, the traditional method of rice variety improvement has relied on sexual hybridization, including intra-variety, inter-variety, inter-subspecies and inter-specific hybridization, as well as the discovery and utilization of new excellent germplasm resources. [0003] In order to further increase rice production and meet the needs of my country's ever-increasing population, my country has im...

Claims

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Application Information

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IPC IPC(8): A01G22/22A01H1/00A01H4/00C12N5/04C12N5/10C12N15/52
Inventor 黄大年钱前华志华高振宇滕胜曾大力卢德赵颜美仙赵艳朱雪峰
Owner CHINA NAT RICE RES INST
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