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Multi-port separation apparatus and method

An outlet device, separation chamber technology, used in separation methods, solid separation, measurement devices, etc.

Inactive Publication Date: 2004-01-14
GRADIPORE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Gradiflow TM technology has advantages, but the technology still has limitations because it employs two separate separation tanks and electrode chambers separated by an electrophoretic separation membrane that practically prevents components between adjacent chambers convective mixing

Method used

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  • Multi-port separation apparatus and method
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  • Multi-port separation apparatus and method

Examples

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Embodiment example 1

[0190] A device according to the invention comprising twelve separation chambers was used to separate immunoglobulin G (IgG) from human plasma. This example illustrates how the device can be used to process the same supplied sample from the same sample container through four sets of identical, multiple, parallel separation chambers.

[0191] Assembly of the separation unit is described below. All ion permeable barriers are polyacrylamide films with different nominal molecular mass (NMM) cutoffs. The first set of parallel separation chambers starts at 1 between the anode chamber and the first separation chamber st Ion-transmissive barrier with NMM of 5,000 Daltons passing through 1 st and 2 nd Another barrier between the separation chambers with NMM of 100,000 Daltons, then 2 nd and 3 nd Another barrier between the separation chambers having an NMM greater than 1,000,000 Daltons. The second set of parallel separation chambers starts at 3 rd and 4 th A barrier between th...

example 3

[0200] A device comprising twelve separation chambers according to the invention is used to separate egg white components according to size. This example illustrates how the device can be used to obtain size-based separations by employing a series of ion-permeable barriers with different nominal molecular mass cut-offs.

[0201] Assembly of the separation unit is described below. All ion permeable barriers are polyacrylamide (NMM) films with different nominal molecular mass cut-offs. Anode chamber with 1 stThe ion-permeable barrier between the separation chambers is a polyacrylamide membrane with an NMM of 3,000 Daltons. 1 st and 2 nd The barrier between the separation chambers has a 5,000 Dalton NMM, 2 nd and 3 rd Barrier between separation chambers with 5,000 Dalton NMM, 3 rd and 4 th The barrier between the separation chambers has a 100,000 Dalton NMM, 4 th and 5 th Another barrier between the separation chambers, which has a NMM of 150,000 Daltons, 5 th and 6 t...

example 4

[0204] A device comprising twelve separation chambers according to the invention was used to separate alpha-1-antitrypsin (ATT, 51 kDa, pi=4.8) from human serum albumin (HSA, 66.5 kDa, pi=4.9). This example demonstrates how the device and Bier buffer can be used to perform quasi-isoelectric focusing separation of components with similar pI values ​​in shallow pH gradients resulting from binary mixing of weak electrolytes.

[0205] Assembly of the separation unit is described below. All ion permeable barriers are polyacrylamide (NMM) films with two different nominal molecular mass cut-offs. anode chamber and 1 st between separation chambers, and 12 th The ion permeable barrier between the separation chamber and the cathode chamber has an NMM of 5,000 Daltons. 1 st and 2 nd ,2 nd and 3 rd , 3 rd and 4 th , 4 th and 5 th , 5 th and 6 th ,6 th and 7 th , 7 th and 8 th ,8 th and 9 th ,9 th and 10 th , 10 th and 11 th , last 11 th and 12 th All other barrier...

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PUM

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Abstract

Multi-port electrophoresis apparatus comprising: (a) cathode chamber with inlet and outlet means defining an anolyte flow path; (b) anode chamber with inlet and outlet means defining an anolyte flow path, to generate an electric field; (c) at least one electrolyte reservoir in fluid communication with the cathode and anode chambers; (d) means adapted for communicating electrolyte between the lectrolyte reservoir and the cathode and the anode chambers; (e) at least three separation chambers adjacently disposed between the cathode and the anode chambers, the separation chambers formed by a plurality of ion-permeable barriers, adapted to impede convective mixing of contents of adjacent chambers; (f) inlet and outlet means for communicating liquid into and out of the separation chambers; (g) at least one sample reservoir, wherein each sample reservoir is connected with at least one separation chamber; and (h) means for communicating fluids between a sample reservoir and at least one separation chamber.

Description

technical field [0001] The present invention relates to a device for separating components in a solution by an electrophoretic separation method, a separation unit and a cartridge suitable for the device, and a method of using the device. Background technique [0002] Preparative-scale electrophoretic separations are important for the processing of both simple and complex samples. An important factor in determining the success of this separation is the degree to which convective remixing of the separated components is prevented. Multi-compartmented electrolyzers are believed to be advantageous for preparative electrophoretic separations because the separated components of a sample can be easily separated spatially and / or temporally. Many multi-compartment electrolyzers have the problem of inappropriate combination of electrophoresis track and hydraulic transport track. Recently, Gradiflow has been adopted TM technology (US5039386, Gradipore Ltd.), which advantageously com...

Claims

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Application Information

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IPC IPC(8): B01D57/02B03C5/00G01N27/447
CPCB01D57/02G01N27/44795G01N27/44769
Inventor 戴维·奥格尔久洛·维格丹尼斯·布雷恩·里拉特
Owner GRADIPORE
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