Spectific binding analyzer and method of analyzing specifie binding
A technology of specific binding and analysis device, which is used in measurement devices, analysis materials, material inspection products, etc.
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Embodiment 1
[0170] According to use Figure 1-Figure 3 The above-mentioned embodiment 1 of the specific binding assay device was carried out in this example. Additionally, using the figure 2 Band of 5 units shown. The monoclonal antibody against hCG obtained by participating in the sandwich reaction with hCG is used as the first specific binding substance and the second specific binding substance, and gold colloid is used as the labeling member to directly label the first specific binding substance. Urine to which a known amount of hCG was added was used as a sample, and the intensity of the signal obtained by the photodetector 8 when the detection unit 6 was irradiated with light of 520 nm was measured to quantitatively measure the concentration of hCG in the sample. Urine with an hCG concentration of 50 IU / L, 100 IU / L, 250 IU / L, 500 IU / L or 1000 IU / L was used as a sample. These samples were introduced from the inlets 3 of the five units.
[0171] In addition, the introduction of th...
Embodiment 2
[0174] According to use Figure 1-Figure 3 The above-mentioned embodiment 2 of the specific binding assay device was carried out in this example. Also, a belt with 2 units is used here. The monoclonal antibody against hCG obtained by participating in the sandwich reaction with hCG is used as the first specific binding substance and the second specific binding substance, and gold colloid is used as the labeling member to directly label the first specific binding substance. Using urine to which a known amount of hCG was added was used as a sample, and the signal intensity at 520 nm was measured for the detection part. Urine with an hCG concentration of 100 IU / L, 500 IU / L, 1000 IU / L, 1500 IU / L, 2000 IU / L or 2500 IU / L was used as a sample.
[0175] In addition, the introduction of the sample is detected by the measurement start detector 10 based on the change in conductivity in the developing layer 1, and the measurement time is measured by setting this time to 0. The holding a...
Embodiment 3
[0184] According to use image 3 , 6 In accordance with the above-mentioned embodiment 3 of the specific binding assay device of 7, this example was carried out. in addition, Figure 7 The number of units in is 5, and here the number of units is set to 6. The monoclonal antibody against hCG obtained by participating in the sandwich reaction with hCG is used as the first specific binding substance and the second specific binding substance, and gold colloid is used as the labeling member to directly label the first specific binding substance. Here, an equal amount of anti-hCG monoclonal antibody solution as the first specific binding substance was enclosed in all the space forming parts 2 . Using urine to which a known amount of hCG was added was used as a sample, and the signal intensity at 520 nm was measured for the detection part.
[0185] Here, urine having an hCG concentration of 100 IU / L, 500 IU / L, 1000 IU / L, 1500 IU / L, 2000 IU / L or 2500 IU / L was used as a sample. Th...
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Abstract
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