Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Technical method for extracting oridinin from rabdosia rubescens

A technology of oridonin and process method, which is applied in the field of extracting oridonin, which can solve the problem that it is difficult to obtain pure oridonin, tannins and polysaccharides cannot be removed well, and Eliminate oridonin and other problems, achieve the effect of saving elution time, saving solvent consumption, and improving product purity

Inactive Publication Date: 2004-09-15
EAST CHINA UNIV OF SCI & TECH
View PDF0 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] These extraction processes usually use solution extraction, then decolorize with activated carbon, concentrate and then directly carry out column chromatography separation. This process cannot remove tannins and polysaccharides well, nor can it remove the tannin and Rubescensin A well. Oridonin B with a molecular weight difference of only 2, it is difficult to obtain pure Oridonin A

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Technical method for extracting oridinin from rabdosia rubescens
  • Technical method for extracting oridinin from rabdosia rubescens
  • Technical method for extracting oridinin from rabdosia rubescens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Extract 1 kg of raw materials, add 6 times the amount of 95% ethanol, extract for 4 hours, pour out the solution, add 4 times the amount of 95% ethanol and reflux for 2 hours, concentrate under reduced pressure, recover the ethanol to obtain a green-brown paste, add 3 times the amount of water and reflux 1.5 hours, filter, pass the filtrate through a macroporous adsorption resin (such as HZ-841 macroporous adsorption resin) column polymerized with styrene and divinylbenzene as monomers, first wash with about 6 times the amount of water until nearly colorless, and then use About 4 times the amount of 60% ethanol solution was eluted, and the eluate was concentrated under reduced pressure to obtain about 12 g of khaki powder.

[0035] Column chromatography separation Mix the khaki powder obtained in the previous step with an appropriate amount of silica gel, and pack the column with silica gel and magnesium oxide (15:1) equivalent to 5 times the amount of the powder. . El...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention advances a new technique proper to separate and purify oridonin. It adopts alcohol solution extraction, then makes water float reflux treatment, where the water float liquor directly passes through the column of macroporous resin (like HZ-841 macroporous absorbing resin) polymerized of styrene and divinylbenzene as monomers to eliminate sugar, pigment, etc, adopts silica gel and magnesium chloride mixed column chromatography to separate and elute, and finally recrystallize to obtain higher purity oridonin. It is safe, low-cost, and applied to industrialized production.

Description

technical field [0001] The present invention relates to a new process method for extracting Rubescensine A from Rubescens. Background technique [0002] Drug research shows that Rubescens has good anti-inflammatory, antibacterial and analgesic effects, and can effectively inhibit type A and B hemolytic streptococcus, Staphylococcus aureus and so on. It has obvious cytotoxic effect on Hela cells cultured in vitro, human esophageal cancer 109 cells and liver cancer BEL-7402 cells. 180 , P 388 , L 1210 Liver cancer and AKS have obvious anti-tumor effects, and Rubescensine A has a mild inhibitory effect on esophageal epithelial hyperplasia. Among them, Rubescensine A, the diterpenoid component, is an effective anti-tumor component, so it is known as "penicillin" and "natural antibiotic" in traditional Chinese medicine. And the United States is also studying the use of Rubescens for the prevention and treatment of AIDS. The development of Rubescensus will become the "sunrise...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07D311/02
Inventor 张立国倪力军谢国祥
Owner EAST CHINA UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com